基于二氧化锆纳米粒子固定乙酰胆碱酯酶的甲基对硫磷-传感技术学报.pdf

第 22 卷 　第 5 期 传 感 技 术 学 报 Vol . 22 　N o . 5 2009 年 5 月 CHIN ES E J OU RNAL O F S EN SORS AND AC TUA TORS May . 2009 Studies on a Novel ParathionMethyl Biosensor Design Based on Acetylchol inesterase Immobil ized by Zirconia Nanoparticle 1 1 1 2 2 L I U S h uj uan , TA N Z hengch u , Z H ON G X i ngg ang , W U Z ais heng , S H E N Guol i 1. H unan Tea R esearch I ns t i t ute , H unan A ca demy of A g ricul t u ral S cience , Changs ha 4 10 125 , Chi na; 2 . Chem ical an d Chem is t ry I ns t it ute , H unan Uni vers ity , Changs ha 4 10082 , Chi na Abstract :A novel bio sen sor for det ecting or ganop ho sp horu s ha s been p rep ared . A zirconia film i s elect ro depo sitio n onto t he cleaned surface of gol d elect ro de . Then acet ylcholine st era se can be immobilized on t he se zirconia nanop article s by adsorp tion . Put ting t he elect ro de into or ganop ho sp hor u s solutio n of diff er ent concent ration s , we can quantit atively det er mine t he or ganop ho sp hor u s concent ration accor din g to t he elect rochemical re spon se int en sit y of t he mo dified elect ro de in t he sub st rat e of acet ylt hiocholine chloride solution . Taking t he p arat hionmet hyl a s examp le , t he eff ect s of sub st rat e concent ration , app lied pot ential an d solutio n p H value on t he analytical p erfor mance have been inve stigat ed , and t he re spon se charact er , rep ro ducibilit y and int erference s of t he bio sen sor are al so t e st ed . The p rop o sed bio sen sor in op timal condi tion s ha s a linear range of 5 . 0 ×10 - 7 ～5 . 0 ×10 -