GSK-3β对子宫内膜样腺癌细胞增殖和侵袭的影响.pdfVIP

· 288· 肿瘤2008年4月第28卷第4期 Tumor Vo1．28，No．4，April，2008 DOI：10．3781／j．issn．1000-7431．2008．04．004 】8‘asicResI潮．cl 基础研究 GSK-31~对子宫内膜样腺癌细胞增殖和侵袭的影响 赵明智，张英丽，刘颖涛，金鸿雁，曹 琦，丰有吉 (复旦大学附属妇产科医院妇产科，复旦大学上海医学院妇产科学系，上海 200011) [摘要] 目的：研究糖原合成酶激酶一3B(glycogen synthase kinase，GSK．3B)对子宫内膜样腺癌已分化细胞株Ishikawa、 HEC-1一A及未分化细胞株KLE增殖和侵袭能力的影响。方法：用GSK．3B小分子干扰RNA(GSK．3B siRNA)转染上述3种细 胞株，采用Western印迹法检测GSK一3B蛋白及凋亡相关蛋白caspase一3的表达；Brdu掺入实验检测细胞增殖率；FCM法检测细 胞周期及凋亡率；Transwel|侵袭试验检测对细胞侵袭、运动能力的影响；明胶酶谱法检测细胞分泌基质金属蛋白酶．2(MMP-2) 的情况。结果：转染GSK一3B siRNA后，3种细胞株中GSK-313蛋白的表达均低于对照组(P0．05)；Ishikawa、HEC．1．A细胞 Brdu掺入率降低，s期细胞数比例减少，凋亡率增加，caspase．3表达上调；细胞侵袭能力降低，与对照组相比差异有统计学意 义(P0．O1)。但是KLE细胞株与对照组相比，增殖和侵袭能力均无明显差异(均为P0．05)。结论：GSK-313能促进已分 化的子宫内膜样腺癌细胞株Ishikawa、HEC一1一A的增殖和侵袭；但是对未分化的KLE细胞株的增殖和侵袭能力无明显影响。 [关键词] 子宫内膜样肿瘤；糖原合成酶激酶3；RNA，小分子干扰；细胞增殖；肿瘤侵润 [中图分类号] R737．33 [文献标志码] A [文章编号] 1000-7431(2008)04-0288-05 Effect of GSK-3[5 on proliferation and invasion of endometrioid adenocarcinoma ZHAO Ming·zhi，ZHANG Ying-li，LIU Ying—tao，JIN Hong·yan，CAO Qi，FENG You—ji (Department of Gynecology and Obstet— rics，Gynecologic and Obstetric Hospital Affiliated to Fudan University，Department of Gynecology and Obstetrics，Shanghai Medical College，Fudan University，Shanghai 20001 1。China) [ABSTRACT] Objective：To investigate the effects of glycogen synthase kinase-313(GSK-313)on the proliferation and invasion abili- ties of the three cell lines with endometrioid adenocarcinoma Ishikawa，HEC．1 A(differentiated)and KLE(undifferentiated)． Methods：Three human endometrioid adenocarcinoma cell lines Ishikawa．HEC．1A．and KLE were transfected with GSK．313 siRNA． The expression of GSK-3 13 protein and apoptosis—related protein caspase一3 were examined by Western blotting．The proliferation of cells transfected with GSK-3 8 siRNA or negative control siRNA were meausred with BrdU incorporation assay while cell cycle distribution and apoptotic ratio