TACE在小鼠动情周期的表达规律.pdfVIP

一 41 6一 重庆医科大学学报2008年第33卷第4期 (Journal of Chongqing Medical University 2008．Vo1．33 No．4) 文章编号：0253—3626(2008)04—0416—03 TACE在小鼠动情周期的表达规律 杨洁玉 ，李 彤2杨 戎 ，谢 怡t，魏莎莉 ，郗 强 (1．重庆医科大学公共卫生学院生殖生理教研gg；2．重庆市人口与计划生育委员会，重庆 400016) 【摘 要】目的：初步探讨肿瘤坏死因子 转换酶Tumor necrosis factor— converting enzyme，TACE)在小鼠动情周期中的表 达规律。方法：用RT—PCR及免疫组化方法分别检测动情周期各期小鼠子宫内膜TACEmRNA和蛋白的表达。结果： TACEmRNA在动情周期小鼠子宫内膜呈规律性表达，在动情期表达明显增强，与其它3期相比有显著性差异(PO．01)；其它 3期间无显著性差异(PO．05)。免疫组化分析显示TACE在子宫内膜的表达规律与RT—PCR一致。结 ：TACE通过裂解EGFR 家族多种配体释放其胞外区，从而激活EGFR信号途径，在子宫内膜的周期性变化中起着重要作用。 【关键词】TACE；子宫内膜；动情周期；RT—PCR；免疫组化 【中国图书分类法分类号】R394 【文献标识码】A 【收稿日期】2007—10—22 Expression pattern of TACE in mouse endometrium during the estrus cycle YANG 一似，et ol (Department of Reproductive Physiology，College of Public Health，Chongqing Medical University) 【Abstract】Objective：To primarily investigate the expression pattern of tumor necrosis factor- converting enzyme(TACE)in mouse endometrium during the estrus cycle．Methods：Reverse transcription polymerase chain reaction(RT—PCR)and immunohistochemistry were used to detect the expression of TACE mRNA and protein in mouse endometrium during each stage of the estrus cycle．Results：The expression of TACE mRNA in mouse endometrium during estrus cycle had regular pattern． The TACE mRNA／13一actin mRNA expression in estrus stage was higher than those of the other 3 groups，and there was significant difference between estrus stage and the other 3 groups(PO．01)．Besides．there was no significant difference among the other 3 groups(P0．05)．Immunohistochemical analysis showed the salne expression pattern of TACE in endometrium as RT—PCR．Conclusion：TACE may play a key role in cyclic changes of normal endometrium through proteolytic removal of the extracellular domain of multiple ligands of EGFR fam ily to release their extracellular doma