Livin反义寡核苷酸对肺腺癌A549细胞增殖的影响 - 江苏大学.PDF

20 4 ( ) Vo.l 20 No. 4 2010 7 Journal of Jiangsu Un iversity( M ed icine Ed ition) July 2010 L iv in A 49 1 1 1 2 1 宋 萍 , 郑金旭, 管淑红, 邵启祥 , 李 坚 ( 1. , 212001; 2. , 212013) [ ] : L iv in ( antisense oligodeoxynucleotides, ASODN )A 49 : L iv in ASODN A 49, - ( RT-PCR )L iv in mRNA; MTT: ASODN A 49L iv in mRNA , L iv in ASODN A 49, , (m issense oligodeoxynu- cleotides, M SODN) (P 0. 0 ): L iv in A 49 L iv in mRNA, , L iv in [ ] ; ; L iv in [ ] R734. 2 [ ] A [] 1671- 7783( 2010) 04- 0342- 03 Effects antisense oligodeoxynucleotides ofL ivin mRNA on the growth of hum an lung adenocarcinom a A549 cells 1 1 1 2 1 SON G P ing , ZH EN G J in-xu , G UAN Shu-hong , SHAO Q i-x iang , LI J ian ( 1. Departm ent ofR espiratory, the A ffiliatedH ospital of JiangsuU niversity, Zhenjiang Jiangsu 212001; 2. Departm ent of Imm unology, School ofM edical Science and LaboratoryM edicine, Jiangsu University, Zhenj iang Jiangsu 212013, China) [Abstract] Objecti e: To explore the effects antisense oligodeoxynucleotides( ASODN) of L iv in mRNA on the proliferation of human lung adenocarcinomaA 49 cells. M ethods: L iv in ASODN w as transfected in- to A 49 cells mediated by lipofectam ine 2000. The expressions of L iv in mRNA was detected by RT-PCR. The proliferation rates ofA 49 cellsw ere assessed by theMTT assay. Results: Itw as observed that the ex- pressions ofL iv in mRNA in ASODN group w ere decreased in a concent