一株琼胶酶产生菌的筛选与鉴定-中国农学通报.PDF

中国农学通报 2013,29(21):88-91 Chinese Agricultural Science Bulletin 一株琼胶酶产生菌的筛选与鉴定 缪伏荣 （福建省农业科学院畜牧兽医研究所，福州350013） 摘 要：利用琼胶作唯一碳源，从腐烂的紫菜中分离筛选到1株高酶活力的琼胶降解细菌Z705。对其菌 落形态、生理生化性质和酶活进行了初步研究，并克隆其16S rDNA 序列，进行分子鉴定。结果表明：该 菌株为革兰氏阴性，呈短杆状；与模式菌株嗜麦寡养食单胞菌Stenotrophomonas maltophilia(AB008509) 的同源性为99% ；根据表型特征、生理生化特性和分子鉴定将菌株Z705 鉴定为寡养单胞菌属 (Stenotrophomonas sp.)。 关键词：琼胶酶产生菌；筛选；鉴定；寡养食单胞菌属 中图分类号：Q939.96 文献标志码：A 论文编号：2012-3571 Screening and Molecular Identification of a New Agarase-producing Bacterium Miao Furong (InstituteofAnimalHusbandryandVeterinaryMedicine,FujianAcademyofAgriculturalSciences, Fuzhou 350013) Abstract: Agar as the sole carbon source, to separation the screening plant height enzyme agarolytic bacteria Z705 from rotting seaweed. The colony morphology, physiological and biochemical properties and enzyme activity was studied, and the clone its 16 s rDNA sequence, molecular identification. The results showed that Z705 was gram negative, a short rod; strain Z705 displayed a high sequence similarity with Stenotrophomonas maltophilia (99%). According to its morphological, physiological and biochemical properties and the homology analysis of 16S rDNA sequence, strain Z705 was identified as Stenotrophomonas sp. Key words: agar-decomposing bacteria; isolate; identification; Stenotrophomonas sp. 0 引言 能，但是长期以来人类未能很好地加以开发利用，其中 [7] 琼胶（琼脂）是存在于琼胶型红藻细胞壁中的一类 一个重要的原因就是未能找到有效降解琼脂的途径 。 多糖，是自然界中存在的重要碳源之一，是中国产量最 利用琼胶酶水解琼胶生产琼胶寡糖的技术被认为是当 大的海洋三大多糖（褐藻胶、琼胶、卡拉胶）之一，广泛