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國立高雄海洋科技大學水產養殖系 學士論文 以光電池測量周氏扁藻Tetraselmis chui的成長 Measuring growth of Tetraselmis chui with a photocell 指導老師：蕭世民博士 學生：林千加、陳昀佐、連子揚 2007年 5月 3日 目錄 Measuring growth of Tetraselmis chui with a photocell 1 摘要 2 Abstract 3 前言： 4 材料與方法： 5 結果： 7 實驗(一) 7 四、 討論 12 摘要 為方便觀察藻類在培養過程中的成長狀況，本實驗在周氏扁藻成長的過程，除計算單位體積藻類個數的同時，並使用一個三用電表測量單一個 _V的光電池因培養液透光度變化所引起的光電池電壓變化。實驗過程計數此電壓變化是否可精準而方便的代表藻類的成長。若此代表性高，此簡便的電壓測量，可用於藻類培養エ作的簡易化及標準化，也有助於將此藻類培養エ作自動化。 實驗一，周氏扁藻在6天以1個空白及3個肥料濃度培養，結果顯示電壓值與藻數的負相關。電壓值(y)與藻數(x)的83對數值間之關係，具y=-0.0016x+9.1806的線性關係(R2=0.71)。實驗二，周氏扁藻在6天以1個空白及2個肥料濃度培養，結果顯示電壓值與藻數的負相關。結果也顯示電壓值與藻數的負相關，電壓值(y)與藻數(x)的83對數值間之關係，具y=-0.0018x+9.3966的線性關係(R2=0.77)。實驗一及實驗二的結果合併，電壓值(y)與藻數(x)的166對數值間之關係，具y=-0.0014x+9.1653的線性關係(R2=0.66)。 此實驗顯示，以容易測量的電壓值推算費時費力始能計算的藻數，此線性公式有超過百分之70的代表性。若使用解剖顯微鏡測量藻類，每一次測量藻類的時間大約10-20分鐘，如果使用此簡易的光電測量則不用3分鐘即可推算1c.c藻水中的藻數。使用此簡易的光電測量技術確實有助於在藻類的培養。  Abstract Over the growth of the alga Tetraselmis chui in fertilized and lighted culture media, the increase of algal cells reduces the light transparency of the culture. In 2 experiments, the growth was measured by cell counting while the corresponding light transparency of each culture was measured as voltage produced by a photocell receiving its passing light. In Experiment 1, over 6 days of culture and with 1 blank and 3 levels of fertilization, 83 pairs of cell count (x) and correspondent voltage (y) were recorded. The relation between these 2 factors is represented linearly as y=0.0016x+9.1806(R2=0.71). In Experiment 2, over another 6 days of culture and with 1 blank and 2 levels of fertilization, another ___ pairs of cell count (x) and correspondent voltage (y) were recorded. The relation between these 2 factors is represented linearly as y= y=-0.0018x+9.3966 (R2=0.77). With these 2 sets of data combined, 166 pairs of cell count (x) and correspondent voltage (y) are available for establish the relation between cell count (x) and correspondent voltage (y). The results is y=-0.0014x+9.1653 (R2=0.66). With the linear relatio