克氏伪裸头绦虫成虫染色标本的制作及其形态学观察-中国农学通报.PDF

中国农学通报 2012,28(21):248-252 Chinese Agricultural Science Bulletin 克氏伪裸头绦虫成虫染色标本的制作及其形态学观察 韩利方，闫文朝，王天奇，武 雷，王培岭 （河南科技大学动物科技学院动物检疫实验室，河南洛阳471003 ） 摘 要：为了弄清克氏伪裸头绦虫的形态结构特征。用苏木素染色和齐尔石碳酸复红染色制作了猪源克 氏伪裸头绦虫成虫染色标本，并对其进行形态学观察。结果显示：颈节、幼节和成节用15倍苏木素稀释 染液染色12 h 效果最佳，孕节以齐尔石碳酸复红染液染色24 h 为最佳。克氏伪裸头绦虫成虫呈扁平状 乳白色，所有节片纵轴小于横轴，从幼节到成节开始逐渐出现睾丸、贮精囊、阴茎囊、卵巢、卵黄腺、子宫 和生殖孔等生殖器官。孕节内充满虫卵，睾丸、卵巢、贮精囊等器官退化，生殖孔未退化。研究结果补充 了寄生虫实验教学标本库，为猪和人克氏伪裸头绦虫病控制提供了重要参考依据。 关键词：猪；克氏伪裸头绦虫；苏木素染色；齐尔石碳酸复红染色；形态学观察 中图分类号：S855.9+9 文献标志码：A 论文编号：2011-3786 PreparationofStainedSpecimensfortheAdultofPseudanoplocephalacrawfordi andItsStructuralObservation Han Lifang, Yan Wenchao, Wang Tianqi, Wu Lei, Wang Peiling (LaboratoryofAnimalQuarantine,CollegeofAnimalScienceandTechnology, HenanUniversityofScienceandTechnology, Luoyang Henan 471003) Abstract: To reveal the characteristic structures of Pseudanoplocephala crawfordi, the stained specimens of the adult of P. crawfordi from pigs were prepared with Hematoxylin staining and Ziehl phenol-basic fuchsin staining. The results showed that the optimal staining condition for collum segment, immature and mature proglottids was in the 1:15 diluted hematoxylin dye solution at room temperature (RT) for 12 hours, and that of gravid proglottids was in Ziehl phenol-basic fuchsin dye solution at RT for 24 hours. Adults of the parasite was flat and white, the vertical axises of proglottids were shorter than the lateral ones. Testicles and sperm pocket began to show in the immature proglottids, and while the complete male and female organs of generation including testicles, sperm pocket, penis pocket, ovary, yelk gland, uterus