响应面试验优化固体酸催化剂催化玉米皮半纤维素水解工艺-食品科学.PDF

14 2016, Vol.37, No.16 食品科学 ※工艺技术 响应面试验优化固体酸催化剂催化 玉米皮半纤维素水解工艺 赵 玮，周瑾琨，王大为* （吉林农业大学食品科学与工程学院，吉林长春 130118 ） 摘  要：采用SO2 －/Fe O / -Al O 负载型固体酸催化剂，催化玉米皮半纤维素水解，对反应工艺条件进行研究。选γ 4 2 3 2 3 择催化剂用量、水解温度、水解时间、料液比进行单因素试验，在此基础上采用Box-Behnken试验设计，以戊糖收 率为响应值进行响应面分析试验，确定最优工艺参数。结果表明：各因素对戊糖收率的影响主次顺序为：催化剂 用量＞水解温度＞水解时间＞料液比；通过响应面法优化并修正得最佳工艺条件为催化剂用量12 mL/10 g玉米皮、 水解温度120 ℃、水解时间4 h 、料液比1∶10，在此条件下戊糖最高收率为22.45%，与理论预测值基本一致。高效 液相色谱分析结果表明，玉米皮水解液主要由葡萄糖、木糖、阿拉伯糖3 种单糖组成，比例为葡萄糖4.99%、木糖 34.78%、阿拉伯糖55.90%；玉米皮及其水解后残渣红外光谱分析表明，半纤维素中木糖基和阿拉伯糖基结构均发 生了变化，说明半纤维素发生了降解。 关键词：玉米皮；半纤维素；水解；固体酸催化剂；响应面分析法 Optimization of Hydrolysis Conditions of Corn Bran Hemicellulose by Supported Solid Acid Catalyst with Response Surface Methodology * ZHAO Wei, ZHOU Jinkun, WANG Dawei (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China) Abstract: This work reports on the optimization of hydrolysis conditions of corn bran hemicellulose by a supported solid 2- acid catalyst, SO γ /Fe O / -Al O . First of all, the one factor-at-a-time method was used to examine the effects of catalyst 4 2 3 2 3 dosage, hydrolysis temperature, time and material-to-solvent ratio on total sugar content and pentose yield. Subsequently, optimization of the four reaction conditions was performed using response surface methodology based on Box-Behnken experimental design. Results indicated that the optimal conditions for hydrolysis of corn bra