嗜麦芽寡养单胞菌整合子与耐药性的相关研究.PDF

. 146 . 1001-8689(2014)02-0146-05 1 2 1 3 3 1,4 1,4 1,4,* (1 230022 2 230022 3 230032 4 230022) (SMA)(Int)qacE 1-sul1 683PCR qacE 1-sul1 intIintIIint 83/ 63.8%75.9%/ 31.3% 18qacE 1-sul120intI 3aacA4-catB8-aadA1aac(6)-II-blaCARB-8aar-3-dfrA27SMAaar-3-dfrA27 GenBankKC748137 SMA/ I R378 A DOI:10.13461/ki.cja.005317 Correlative research of integrons and antimicrobial resistance among clinical Stenotrophomonas maltophilia isolates 1 2 1 3 3 1,4 1,4 1,4 Gao Li-ping , Hu Li-fen , Yang Hai-fei , Wang Zhong-xin , Shen Ji-lu ,Ye Ying , Xu Xi-hai and Li Jia-bin (1 The First Affi liated Hospital of Anhui Medical University, Department of Infection Diseases, Hefei 230022; 2 The First Affi liated Hospital of Anhui Medical University, Department of Center Laboratory, Hefei 230022; 3 The First Affi liated Hospital of Anhui Medical University, Department of Clinical Laboratory, Hefei 230022; 4 Anhui Center Surveillance of Bacterial Resistance, Hefei 230022) Abstract Objective To investigate the characteristics of antimicrobial resistance, the distribution of integrons and gene cassettes embedded in clinical Stenotrophomonas maltophilia isolates. Methods Minimal inhibitory concentrations of six antimicrobial agents was determined by agar dilution method; The presence of qacE 1- sul1gene, class 1, 2 and 3 integrase as well as gene cassettes embedded within class 1 integron were detected by polymerase chain reaction (PCR). Results Cef