基于生物信息学方法的fox 基因家族对比分析 - researchgate.pdf

See discussions, stats, and author profiles for this publication at: /publication/308874028 Comparative Analysis of FOX Family Genes Based on the Bioinformatics Methods Article · Septe ber 2015 DOI: 10.13417/j.gab.034.001681 CITATIONS READS 0 17 6 authors, including: Kai Chang Chengdu Military General Hospital 11 PUBLICATIONS 29 CITATIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: Medical Genetics and Clinical Molecular Diagnosis View project Metabolic Engineering View project All content following this page was uploaded by Kai Chang on 04 October 2016. The user has requested enhance ent of the downloaded file. All in-text references underlined in blue are added to the original docu ent and are linked to publications on ResearchGate, letting you access and read the i ediately. 基因组学与应用生物学，2015 年，第34卷，第9 期，第 1-8页 GenomicsandAppliedBiology,2015,Vol.34,No.9,1-8 研究报告 ResearchReport 基于生物信息学方法的FOX基因家族对比分析 常凯 熊怡淞 曲远青 吴艾霖 陈清红 吴丽娟* 中国人民解放军成都军区总医院实验医学中心,高湿医学全军重点实验室,成都,610083 *通讯作者,wulijuan1638@126.com 摘 要 以FOXA、FOXO和FOXP为核心，利用生物信息学方法对比分析FOX家族成员的分子特征、启动 子、蛋白理化性质、蛋白结构、亚细胞定位，构建分析蛋白三维模型并绘制家族系统进化树。结果表明：FOX家 族的核酸序列相似性较低，FOXP和FOXN序列内含较多内含子，但仍具有溯源性。蛋白的脂肪族氨基酸指数 介于49.71 到76.11之间，分子量介于29084 到79586.1之间，较为离散。GRAVY值介于-0.764到-0.179之 间，蛋白均表现为亲水性。pI值在亚家族间非常稳定，可作为蛋白分类的辅助指标。对比统计FOX 蛋白高级结 构各亚家族的保守结构域和功能位点，可见功能域的差异导致了其调节途径的多样。分析结果可为FOX蛋白 的研究提供有价值信息，为进一步研究FOX在人体内的疾病发生途径与作用机理提供依据。 关键词 叉头框基因家族,生物信息学,对比分析,分子特征, 蛋白特征 ComparativeAnalysisofFOXFamilyGenesBasedontheBioinformatics Methods ChangKai XiongYisong QuYuanqing WuAilin ChenQinghong WuLijuan* KeyLaboratoryofHighHumidityMedicine,CenterofLaboratoryMedicine,ChengduMilitaryGeneralHospital,Chengdu,610083 *Correspondingauthor,wulijuan1638@126.com DOI:10.13417/j