紫外诱变选育高效降解甲醛菌株及其降解特性 - 食品科学.pdf

※生物工程 食品科学 2015, Vol.36, No.13 143 紫外诱变选育高效降解甲醛菌株及其降解特性 1 2 1 1 1 1 姚璐晔 ，李 想 ，邢 鋆 ，高 杏 ，曹叶萍 ，顾佳佳 （1.常熟理工学院生物与食品工程学院，江苏 常熟 215500 ；2.江南大学生物工程学院，江苏 无锡 214122 ） 摘 要： Paecilomyces variotii 以甲醛降解菌——拟青霉菌（ ）CSLG1为出发菌株，对其进行紫外诱变，并考察突 变株对甲醛的降解特性。选用功率15 W的紫外灯、照射距离30 cm、照射时间30 s、致死率为88%的诱变强度对出 发菌株进行紫外诱变；通过初筛、复筛选育出一株甲醛抗性及其降解能力明显提高的诱变菌株F1-23 。结果表明： F1-23 临界甲醛抗性质量浓度为7.88 g/L ，比诱变前提高22.2%；甲醛脱氢酶酶活力为83.6 U/mg，提高了23.8% 。连 续传代5 代，诱变菌株F1-23 的甲醛抗性和降解力基本不变，证实其具有良好的遗传稳定性。诱变菌株F1-23对甲醛 的抗性及降解能力均较出发菌株高。 关键词：甲醛降解；拟青霉菌；紫外诱变；甲醛脱氢酶；降解率 Ultraviolet Mutagenesis of Paecilomyces variotii for Formaldehyde Degradation and Degradation Characteristics of Its Mutant Strain 1 2 1 1 1 1 YAO Luye , LI Xiang , XING Yun , GAO Xing , CAO Yeping , GU Jiajia (1. School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China; 2. School of Biotechnology, Jiangnan University, Wuxi 214122, China) Abstract: Paecilomyces variotii CSLG1A, a strain able to degrade formaldehyde, was mutagenized by ultraviolet (UV) irradiation and the selected mutants were tested for their abilities to degrade formaldehyde. The UV mutagenesis was carried out by placing the original strain at a distance of 30 cm away from a 15-W UV light lamp for 30 s, yielding a mortality rate of 88%. The mutant stain F1-23 with significantly increased formaldehyde degradation ability was obtained by two rounds of screening. The critical formaldehyde concentration degraded by the mutant