西伯利亚鲟卵黄脂磷蛋白的分离纯化及性质 - 动物学杂志.pdf

Chinese Journal of Zoology 2009,44(3) :9~ 15 * 丁建文 庄 平 章龙珍 侯俊利 张 涛 刘鉴毅 颜世伟 ( 200237; 200090) : , Sephacryl S300 ( Acipenser baerii) (lipovitellin, Lv) , 7 SDS AGE OSchiff , b , b , SDS AGE , 3 , 306 ku408 ku 767 kuLv , , : ; ; ; : Q955 :A : 2009) 030907 Purification and Partial Characterization of Lipovitellin from Acip enser baerii * DING JianWen ZHUANG ing ZHANG LongZhen HOU JunLi ZHANG Tao LIU JianYi YAN ShiWei ( East China University of Science and Technology, Shanghai 200237; East Fishery Research Institute, ChineseAcademy of Fishery Sciences, Shanghai 200090, China) Abstract:The yolk protein is the chief nutrition material during embryogenesis.The quantity and quality of yolk is crucial for early larval growth.We have used Sephacryl S300 gel filtration chromatography to separate lipovitellin from theAcipenser baerii. There were seven protein chromatography peaks. The peak b protein was composed of three subunits with molecular weights of 306 ku, 408 ku and 767 ku, respectively as revealed by the SDS AGE electrophoresis analysis. Oil red O, Methyl green and the Schiff staining showed that the peak b protein was rich in glucose,lipid and phosphorus. Lipovitellin of A . baerii contained rich asparagines acid, lysine acid, glutamic acid, serine acid, leucine acid and valine acid. The quantity of flavor amino acids such as glutamic acid and asparagines acid in A . baerii was abundant