氨基酸分析仪食品及饲料中的应用.ppt

Sample Preparation Applications in food and feedstuff.样品制备-食品和饲料应用 Purpose and Scope 应用目的和领域 Factors to consider 需要考虑的因素 No single hydrolysis method can totally recover all the protein amino acids 没有任何一个水解方法得到的结果能够定量所有的蛋白氨基酸 Oxidation procedure 氧化步骤 Alternative hydrolysis methods for Tryptophan色氨酸的水解方法 Protocol 协议 Oxidation with Performic acid at 0oC for 16h 过甲酸0度16小时水解 Sample containing 10mg Nitrogen 含氮量10mg样品 Hydrolysis in 6N HCl at 110oC for 24h。6摩尔/升盐酸110度水解24小时 Extraction of free AA with loading buffer 用上样液提取游离氨基酸 Deproteinisation 除蛋白 Neutralisation: NaOH 用NaOH 中和 Hydrolysis in 6N HCl at 110oC for 24h 6摩尔/升盐酸110度水解24小时 Neutralisation: NaOH 用NaOH 中和 Combined results give global protein AA content 蛋白中结合氨基酸含量 Analysis 分析 Analysis 分析 Free amino acid content 游离氨基酸含量 Polyamine analysis 多胺分析 Biogenic amines are mostly detrimental and can generally be considered as markers of protein degradation. 生物胺多是有害的，且通常是蛋白降解物 in large doses histamine can cause food poisoning and become toxic. 大量的组胺可导致食物中毒或称为有毒物质 According to the 91/493/EEC directive the average level of histamine in fish should not exceed 100 mg/kg. 按照91/493/EEC指令，鱼肉中组胺平均水平不超过100mg/kg。 Requires a specific column and buffer to run on the Biochrom AAA 在BiochtomAAA上需要特定分离柱和缓冲液分析 Analysis of Protein Hydrolysates on Lithium Systems在锂系统中分析蛋白水解样品 Arg elutes in 60 min (total analysis time 93 min) 净分析时间60分钟（全分析时间93分钟） Standard 标准品 Hydrolysate 水解样