巨刺法对大鼠脑缺血再灌注损伤的神经保护机理的探析 - scholarmate.doc

电针对缺血性脑卒中再灌注损害大鼠CREB表达的影响巨刺针法改善脑卒中后肌肉低张状态的机理研究20103519110001）教育部博士点基金 博导类 2、巨刺法调控G-蛋白信号转导影响GABA受体表达改善缺血性脑卒中后肌张力的机理研究 青年科学基金项目 作者单位：1:杨珊莉 福建中医药大学附属康复医院 1 江一静 福建中医药大学 2陶静 福建中医药大学附属康复医院 通讯作者：陈立典 福建中医药大学 摘要 目的：探寻电针对缺血性脑卒中再灌注损害大鼠CREB表达的影响。方法：将60只健康成年的Sprague Dawley大鼠（SD鼠）将动物驯养3天随机分成假手术组、模型组以及电针组，每组各20只，使用线栓法（MCAO）对模型组、电针组大鼠进行脑缺血模型制作，缺血2h后进行再灌注，电针组大鼠接受电针干预，再灌注3d后将大鼠断头取脑，进行TTC染色以及图像软件分析观察脑梗死体积，蛋白印迹法（Western blotting)检测CREB蛋白水平，荧光PCR检测CREB的基因表达水平。结果：（1）TTC及其图像软件分析：电针组的SD大鼠脑梗死面积明显小于模型组（P0.01）。（2）Western blotting：电针组的SD大鼠CREB蛋白水平明显高于模型组（P0.01）。荧光PCR：电针组的SD大鼠CREB基因水平明显高于模型组（P0.01）。结论：电针改善脑缺血再灌注损伤可以通过上调CREB实现。 关键词 脑缺血再灌注损伤lectro-acupuncture on the expression of CREB in ischemia-reperfusion rats Shanli Yang1, Yijing Jiang1, Jing Tao2, Lidian Chen3 Shanli Yang , Rehabilitation Hospital affiliated to Fujiang University of Traditional Chinese Medicine, Yijing Jiang, Fujian University of Traditional Chinese Medicine, 2. Jing Tao, Rehabilitation Hospital affiliated to Fujian University of Traditional Chinese Medicine, 3. Lidian Chen, Fujian University of Traditional Chinese Medicine, [Abstract]Objective: to explore the impact of electro-acupuncture on the expression of CREB in the ischemia-reperfusion rats. Methods: randomly divided 60 healthy adult Sprague Dawley (SD rats) rats (which were domesticated for 3 days) into the shame group, the model group and the electro-acupuncture group, 20 rats per group, used the line bolt method (MCAO) on the model group and the electro-acupuncture group to complete the brain ischemia model, reperfused the rats 2 h after ischemia. The electro-acupuncture group was adopted the intervention of electro-acupuncture. 3 d after reperfusion, all the rats were beheaded and took brains, observed the cerebral infarction volume by TTC staining and image analysis, the protein expression of CREB were detected by protein imprinting method（Western blotting) and gene expression of CREB were tested by Real-Time PCR. Results: (1