5-氟尿嘧啶及长春新碱对胃癌细胞sgc7901干细胞相关表型和特性的 .doc

5-氟尿嘧啶及长春新碱对胃癌细胞SGC-7901干细胞相关特性的影响 陈 军，余佩武 （400038 重庆，第三军医大学西南医院全军普通外科中心，微创胃肠外科中心R735.2 [文献标志码] A Effects of 5-FU and vincristine on gastric cancer stem cells-associated phenotypes and properties in SGC-7901 Chen Jun,Yu Peiwu（General Surgery Center of PLA, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China ） [Abstract] Objective To identify the effects of 5-FU and vincristine (VCR) on gastric cancer stem cells-associated phenotypes and properties in gastric cancer cell line SGC-7901. Methods SGC-7901 were treated with 5-FU and VCR respectively. Candidate gastric cancer stem cells’ marker CD44 and CD133 were assayed as well as other stemness genes and EMT associated genes. Besides, some assays such as colony formation, sphere formation and tumorigenic formation were used to test the properties associated with cancer stem cells. Results After 5-FU and VCR treatment, the mRNA expression levels of CD44 and CD133 were more in all of the doses in current experiment (up to 2.36±0.03, 1.57±0.07, 6.03±0.7, 4.06±0.76 folds respectively, P＜0.05). But the capability of colony formation and tumorigenic formation didn’t increased. However, after VCR treatment SGC-7901 manifested the EMT gene profile and showed different morphologic features in serum-free culture, which was different from 5-FU treatment. Conclusion Transient treatment of 5-FU and VCR respective promoted the expression of CD44 and CD133. Besides, VCR induced EMT changes of SGC-7901 and enhanced sphere formation in serum-free culture medium. [Key words] gastric cancer stem cells; 5-FU; vincristine; epithelial-to-mesenchymal transition Corresponding author: Yu Peiwu, E-mail:yupeiwu01@ [通信作者] 余佩武，E-mail: yupeiwu01@ 肿瘤干细胞被认为是肿瘤组织中一小部分具有自我更新和多向分化潜能的肿瘤细胞，是肿瘤发生、发展、转移及复发的元凶[1]。虽然胃癌干细胞在多项研究中得以证实，但相对于其它类型的肿瘤干细胞而言，目前对其认识仍比较局限[2-4]。近年来有不少研究关注化疗药物对肿瘤干细胞的影响，认为肿瘤干细胞对化疗药物具有耐受性，据此在一定程度上可富集肿瘤干细胞。而临床上常用的化疗药物如5-氟尿嘧啶（5-FU）和长春新碱（VCR）在常规3～5d化疗后能否直接影响胃癌细胞干细胞相关表型及功能尚不清楚。因此