响应面法优化竹笋剩余物甾醇的超声辅助提取工艺.pdf

※工艺技术 食品科学 2012, Vol. 33, No. 14 85 1 2 1 , 1 陈如寿 ，殷钟意 ，郑旭煦 * ，伍冬婷 (1.重庆工商大学环境与生物工程学院，重庆 400067 ； 2.重庆工商大学药物化学与化学生物学研究中心，重庆 400067) 以方竹笋加工剩余物为原料，以总甾醇、β- 谷甾醇提取率为考察指标，采用超声波处理法提取竹笋甾 醇。在采用单因素试验考察料液比、超声功率、超声时间及颗粒粒径对竹笋甾醇提取率影响的基础上，利用响应 面分析法优化超声波处理法提取竹笋甾醇的工艺，在料液比 1:31、超声功率410W 、超声时间 10.5min、颗粒粒径 86 目条件下，竹笋总甾醇提取率达0.317% ，β- 谷甾醇提取率达 0.249% 。 竹笋剩余物；植物总甾醇；β- 谷甾醇；响应面分析；超声波处理 Optimization of Ultrasonic-Assisted Extraction Process for Phytosterol from Bamboo Shoot Scraps by Response Surface Analysis 1 2 1, 1 CHEN Ru-shou ，YIN Zhong-yi ，ZHENG Xu-xu *，WU Dong-ting (1. College of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067, China；2. Research Center of Medical Chemistry and Chemical Biology, Chongqing Technology and Business University, Chongqing 400067, China) Abstract ：In this study, response surface methodology was used to optimize the ultrasonic-assisted extraction of phytosterol from bamboo shoot scraps. The effects of four variables including solid-to-solvent ratio, ultrasonic power, ultrasonic treatment time and raw material particle size on total phytosterol and β-sitosterol yields were investigated. The optimal extraction conditions were found as follows: 1:31 of solid-to-solvent ratio, 10.5 min of ultrasonic treatment time, 410 W of ultrasonic power, and 86 mesh of raw material pa