胶体金标记牛血清白蛋白及其在电场中的迁移行为 - journal of .pdf

第28 卷第2期 东 北 大 学 学 报 ( 自 然 科 学 版 ) Vol28, No. 2 2 00 7 年 2 月 Journal of Northeastern U ni ersity( Natural Science) Feb. 2 0 0 7 王文星, 陈启凡, 张 丽, 徐淑坤 ( , 110004) : , ( BSA) , BSA , BSA , pH BSA : , pH 58, BSA , ; BSA , BSA : ; - BSA ; ; pH ; : R 318; T B 383 : A : 1005-3026( 2007) 02-030 1-04 Conj ugation of Colloidal Gold to BSA and Its Migration in Electric Field WAN G Wen-x ing , CH EN Qi-f an, ZHANG L i, X U Shu-kun ( School of Sciences, Northeastern Uni ersity, Shenyang 110004, China. Correspondent: XU Shu-kun, Professor, E-mail: xushukun46 @ 126. com) Abstract: Gold nanoparticles w ith 14 nm , 19 nm and 40 nm in diameter were synthesized through citrate sodium reduction method, and all of them w ere labeled w ith BSA. The influencing factors on the conjugation of colloidal gold to BSA were discussed. M igrations of those gold nanoparticles and their Au-BSA conjugates in agarose-gel electrophoresis w ere studied. Experimental results indicated that pH alue, ion concentration and protein addition all ha e influences on the conjugation of colloidal gold to BSA. The optimal conditions for BSA labeling are salt-free or ery low salt concentration and pH alue= 5. 8 and especially BSA addition isn t less than the minimal stabilizing protein concentration. Migratory beha ior of gold nanoparticles and Au-BSA conjugates in the applied electric field is similar to that of rocket immuno-electrophoresis, w here the migration speed decreases w ith increasing gold nanoparticles size. How e er, after the conjugation, the migration speed of the conjugates become