小麦脂质转运蛋白cdna 结构及相应肽链构造特征的分析.pdf

华北农学报2007 , 22( 2) : 1- 5 cDNA 1, 2 2 1 1 2 孟成生 , 李爱丽, 王省芬, 马峙英, 贾继增 ( 1. , , 071001; 2. , , 100081) * : 1 261 bp cDNA , , Mardler/ 7 3217( P m2 ) cDNA 1651 bp cDNA , LTP1 542 bp, 3261 bp, 348 bp, 115 , 11. 2 D, 9.468 ( C) 25 , ( GenBan ) A Y796184 ( ) AAV65513 ( ) / , : ; cDNA ; : S51201 :A : 1000- 7091( 2007) 02- 0001- 05 Sequence Analysis of Wheat Lipid Transfer Protein cDNA and the Structural Characteristics of Corresponding Peptide 1, 2 2 1 1 2 MENG Cheng-sheng , LI A-i li , WANG Xing-fen , MA Zh-i ying , JIA J-i zeng ( 1. Agricultural University of Hebei, Key Laboratory of Crop Germplasm Resources of Hebei, Baoding 071001, China; 2. Key Laboratory of Crop Germplasm and Biotechnology,Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081, China) Abstract:One of the sequenced cDNA fragments was highly identified with LTP1 . The EST was extended using CAP3 software in the lab local net. Based on the extended sequence, a pair of primers was designed in the two side re- * gions of its predicted ORF. Using RT-PCR method, a cDNA fragment of 651 bp was isolated from Mardler/ 7 BaiNong 3217. It was found that this cDNA contained the complete coding sequence of LTP1 of wheat including 42 bp of 5 un- translated region, 261 bp of 3 untranslated region, and an open reading frame ( ORF) of 348