100蛋白在b16 黑色素瘤模型鼠的表达和意义ξ - 现代生物医学进展.pdf

　　　　　现代生物医学进展 　　　Progress in Modern Biomedicine 　　　2006 　Vol. 6 　No. 8 　　　　　 ·24 · nm23 和 S - 100 蛋白在B16 黑色素瘤模型鼠的表达和意义 王忠成 ( 哈尔滨医科大学大庆校区解剖学教研室 　黑龙江 大庆 163319) ( ) 摘要 　目的:检测荷瘤鼠体内 nm23 肿瘤转移抑制因子 与 S - 100 蛋白的表达 ,探讨其表达与肿瘤发生、转移的关系。方法 : 将 B16 恶性黑色素瘤细胞人工植入 C57 小鼠背部皮下 ,制荷瘤鼠模型 ,取对照组和病变皮肤作免疫组化染色。结果 :早期肿瘤 ( ) nm23 和 S - 100 蛋白阳性表达的数密度和面密度明显增高 ,与对照组、晚期组比较差异显著 P 0. 05 。结论 :在 B16 黑色素瘤荷 瘤鼠 nm23 和 S - 100 蛋白的表达与肿瘤的发生、转移呈负相关。 关键词 :nm23 ;S - 100 蛋白 ;黑色素瘤 中图分类号:R739. 5 　文献标识码 :A The Expression and Significance of S - 100 Proteins and Nm 23 in Rats B16 WANG Zhong - cheng ( ) Department of Anatomy , Daqing Branch , Harbin Medical University , Daqing 163319, Heilongjiang , China ABSTRACT Objective : To determine the expression and significance of Nm23 and S - 100 Proteins and explore the relationship between them and tumor ’s forming and metastases. Methods : The cultured melanoma cells were transplanted into the hypoderm of the back of Rats C57 to make rat models. The pathological skins were taken out and were dyed by using method - immunity group. Results : The number density and area density of positive expression of Nm23 and S - 100 Proteins increased obviously. Compared to control group and late tumor group , the differ ence was more significant (P 0. 05) . Conclusion : The expression of Nm 23 and S - 100 Proteins in Rats B16 has negative relevance to the tu