免疫酶吸附法评价纳米金对抗体fc 端的吸附效果 - 食品与生物技术学报.pdf

第29 卷第4 期 食 品 与生 物 技 术学 报 Vol. 29 No.4 2010 年7 月 Journal of Food Science and Biotechnology Jul. 2010 : 1673- 1689( 2010)04-0533-05 免疫酶吸附法评价纳米金对抗体Fc 端的吸附效果 * 汤俊琪, 庞广昌 , 高嘉荣, 梁新义 ( 天津市食品生物技术重点实验室, 天津商业大学, 天津300134) : 以简单快速的微波技术制备 粒径约为15nm 左右纳米金溶胶为测定该纳米金对兔 IgG 抗体的吸附, 扫描得到溶液吸附抗体前后的光吸收图谱, 可以明显看到溶液最大光吸收峰从 518 nm 转移到526 nm,这种现象表明纳米金能够很好地结合抗体为评价纳米金和抗体Fc 端的 吸附效果, 以兔IgG 抗体和酶标羊抗兔抗体反应后, 测定上清液中酶标抗体对酶底物显色后O. D 值的变化, 结果表明, 纳米金对抗体 Fc 端的吸附率在92% 以上, 证明该纳米金吸附主要是在抗体 的Fc 端 : 纳米金; 胶体金; 抗体Fc; 免疫 吸附技术; 吸附 :RS 201; O 657. 32 : A A Study of Immunoenzymatic Techniques of the Adsorption Effect of Nano- Gold to Antibody Fc Part * T ANG Jun-qi, PANG Guang-chang , GAO Jia-rong, LIANG Xin-yi (T ianjin Key laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China) Abstract:In this study, a nano-gold which diameters were about 15 nm was prepared through a the simple and rapid microwave technique. T he nano-gold absorbing rabbit IgG was performed by UV-visible spectrophotometer. The absorption maximum wavelength of gold colloidal solution was obvious changed when IgG coupled to nano-gold. It range from 518 nm to 526 nm. For the adsorption effect of nano-gold to antibody-Fc part, it was described by the variation of O. D. When rabbit IgG reacted with HRP-labeled goat ant-i rabbit IgG and formed a compound, the remaining HRP-labeled goat ant-i rabbit IgG in the supernatant decreased after centrifugation. T he H RP catalyzed substrate reaction with the color variation. Finally it was shown a good adsorption effect through the ratio of O.D,