一阶导数紫外光谱法测定盐酸芬氟拉明片的含量及含量 - 药学实践杂志.pdf

154 The Journ l of Ph rm ceutic l Pr ctice Vol. 18 2000 No. 3 戴叶华, 徐宏祥( , 314001) : : 建立 一阶导数紫外光谱法测定盐酸芬氟拉明片的含量及含量均 度, 以增加质量控制 方法: 用水作溶剂, 在 280 与220nm 波长范围内测定一阶导数光谱, 以 一阶导数光谱在 265nm 与269nm 波长处峰- 谷间的振幅D 为定量依据, 用对照品对照法计算含量及含量均 度 : 盐酸芬氟拉明浓度在005~ 04mg/ ml 之间与 阶导数峰 谷间的振幅呈良好的线性关系, 相关系数r= 09999, 6 次的平均加样回收率为1006% , RSD = 07% : 本法准确性和重现性 好, 操作简便快速 : 一阶导数紫外光谱法; 盐酸芬氟拉明片; 含量测定; 含量均 度 : R9272 : A : 1006- 0111( 2000) 03- 0154- 03 Determination of fenfuramine hydrochloride tablets with first order deriva- tive spectrophotometry DAI Ye-hu , XU Hong-xi ng( Ji xing Institute for Drug Control of Zheji ng province, Ji xing 314001) ABSTRACT:OBJ CTIV :To provide useful w y of qu lity control, first order deriv tive spectropho- tometric method h d been developed to determine the content nd uniformity of content of fenfur mine hy- drochloride t bles. M THODS:W ter w s selected for solvent. The first deriv tive spectrum w s obt ined by sc nning from 280 to 220nm.The dist nce D of the deriv tive pe k t 265nm nd v lley t 295nm w s used s qu ntit tive b sis.The ss y w s c lcul ted by comp ring the pe k- v lley dist nce of s mple with st nd rd. R SULTS:There w s good line r rel tionship between fenfur mine hydrochloride concentr tion in the r nge of 0. 05~ 0. 40mg/ ml nd the pe k- v lley dist nce of deriv tive spectrum with r = 0. 9999. The ver ge re- covery form s mple w s 100. 6% . RSD = 0. 7%( n = 6) . CONCLUSIONS:The proposed method is simple, ccur te nd reproducible. K Y WORDS:first order devi tive spectrophotometry; fenfur mine hydrochloride t blets; qu ntit tive n lysis; uniformity of content ( fenflur mine hydrochloride) , , 1995 , BP1993 1 [ 1, 2] [ 1] , , UV- 265FW GC- [3] [4] , 14B ( ) ;N- 2000 [5] , ( ) : AE- 240 , ( 1/ 100 000 ) ; (