银杏叶萜内酯含量的变化及其与叶绿素荧光特性的关系.pdf

热带亚热带植物学报　2015 ，23(6) ：683 ~ 690 Journal of Tropical and Subtropical Botany 银杏 叶萜 内酯含量的变化及其与叶绿素荧光特性 的关系 a,b* b a 肖强 , 张峥, 周大寨 (湖北民族学院， a. 生物资源保护与利用湖北省重点实验室； b. 林学园艺学院，湖北 恩施 445000) 摘要： 为探究银杏(Ginkgo biloba)叶萜类 内酯含量和光合 同化作用的关系 ，对其 内酯含量和叶绿素荧光特性进行 了研究 。结果 表明，不 同采收时间银杏叶中白果 内酯和银杏 内酯含量有显著差异 ，总体上 ，5月份含量较低 ，此后逐渐升高 ，8月份达到高峰 ， 然后快速下降 ，10月底最低 ；与此 同时 ，银杏叶片的光合色素以及叶绿素荧光参数也呈现周期性变化 。白果 内酯以及萜 内酯含 量与叶绿素荧光参数Y(NPQ)之间呈极显著正相关关系 ，因此 ，可以通过银杏叶片的叶绿素荧光参数预测 白果 内酯和萜 内酯含 量 。 关键词： 液质联用 ；银杏 ；萜 内酯 ；叶绿素荧光 doi: 10.11926/j .issn.1005–3395.2015.06.013 Changes in Terpene Lactones of Ginkgo biloba and Its Relation with Chlorophyll Fluorescence Characters a,b* b a XIAO Qiang , ZHANG Zheng , ZHOU Da-zhai (a. Key Laboratory of Biological Resources Protection and Utilization of Hubei Province; b. School of Forestry and Horticulture, Hubei University for Nationalities, Enshi 445000, Hubei, China) Abstract: In order to understand the relationship between contents of terpene lactones and photosynthetic characters in Ginkgo biloba leaves, the contents of terpene lactones were determined and chlorophyll fluorescence features was studied. The results showed that the changes in terpene lactone contents were significant at different harvest time. In general, bilobalide and ginkgolide contents were low in May, then increased gradually, reached peak in August, and afterwards decreased until the end of October. At the same time, the photosynthetic pigment content and chlorophyll fluorescence parameters in leaves showed periodical changes. The contents of bilobalide and lactone had significant positive correlation with chlorophyll fluorescence index of Y(NPQ). So, it was suggested that bilobalide and lactone contents could be forecasted by chlorophyll fluorescence parameters. Key words: UPLC-MS; Ginkgo biloba; Lactone; Chlorophyll fluorescence 银 杏(Ginkgo biloba)为银 杏科(Ginkgoaceae)银 为 A