羟基磷灰石负载奥沙利铂对癌细胞的影响.pdf

羟基磷灰石负载奥沙利铂对癌细胞的影响 韩敬，廖婕，和祥，王楠，张雪莹 （中南大学粉末冶金研究院，湖南省长沙市 4100001） 刘咏 正高 摘要：论文采用模板辅助-生物仿生矿化法及水热法两种方法制备不同形貌及性能的羟基磷灰石 （HA）材料，应用XRD，扫 描电镜，能谱仪，透射电镜，紫外光度仪，激光粒径分布仪等设备对材料的成分、组织结构、微观形貌和药物装载量等性能 进行分析检测，同时对微纳米羟基磷灰石晶簇与奥沙利铂(OP)药物复合物对直肠癌细胞的影响进行系统研究。 1.应用Nafion 阳离子交换膜辅助构建特异形貌HA 纳米结构：应用美国杜邦阳离子交换膜辅助电沉积技术，通过改善Pt 电极的电势,反应温度等因素来制备三维结构羟基磷灰石晶簇。 2.优化水热法制备球型HA 纳米结构：在水热合成的基础上，通过调节温度，浓度制备出羟基磷灰石纳米晶簇，产率提 高的同时具有球型形貌，为后期实验提供充足的载体。 3.以冷冻干燥法制备药物复合载体：经过震荡摇床处理，离心处理后，迅速冷冻干燥得到HA-OP 复合药物微球。 4.利用MTT 法测定纯奥沙利铂，纯HA，不同浓度HA-OP 复合微球对直肠癌细胞的增殖影响。 关键词：羟基磷灰石 晶簇 药物载体 直肠癌 ABSTRACT:Inthisstudy,biotemplate-assistedbiomineralizationmethodandHydrothermal synthesismethodareusedtocompose the Hydroxyapatitematerialwith different featuresandcapabilities,applyingtheXRD,SEM,TEM,EDS,FIRS,BET,andLPSto analyzeandcheckthecomposition,microstructure,Organizational structureandtheencapsulationrateofthecarrier.Andthestudy systematically investigatesthecomposition ofthemicro/nanohydroxyapatiteandoxaliplatin drugsandtheeffectstotherectal cancer cells. 1.A Nafion cation-exchange membrane assists to build the multi-dimensional HA nano-structure ：The Nafion-assisted electrodeposition technology isusedto compose theHAnanocrystalline cluster with three-dimensional structureby improving factors such astheelectricpotentialofPt andreactiontemperature. 2.The Hydrothermal synthesis method is optimized to compose the spherical nanostructures: On the basis of hydrothermal synthesis,I have composed theHANanocrystalline cluster with spHerica nanostructures andraised itsproductivity by modulatingthe temperatureandconcentration,providingsufficientcarriersfor later experiments. 3.The freeze drying method is used to prepare the drug composite microspheres: HA - OP compound drug microsphere are composedaftertheprocession ofvibratingshakingtable,centrifugal separator,andthen quickly