聚酰胺-大孔树脂联用富集苦参总黄酮 - 天津中草药杂志社.pdf

·1968 · 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 10 期 2011 年 10 月 聚酰胺-大孔树脂联用富集苦参总黄酮 1 1* 2 2 1 张 蕾 ，廖琼峰 ，谢智勇 ，冯志强 ，曾元儿 1. 广州中医药大学中药学院，广东 广州 510006 2. 中山大学药学院，广东 广州 510080 摘 要：目的 寻找一种有效富集苦参总黄酮的方法并对工艺条件进行初步探索。方法 样品溶液以聚酰胺吸附，水洗除杂， 将除杂后的聚酰胺加于大孔吸附树脂顶部，以一定体积分数的乙醇溶液洗脱富集总黄酮。结果 聚酰胺与 AB-8 大孔树脂联 用能有效富集总黄酮，且可与其生物碱类成分有效分离。最佳工艺条件为浸膏与聚酰胺比为 1 ∶1，与树脂比为 1 ∶3，以80% 乙醇洗脱，收集洗脱液 3 BV，富集总黄酮质量分数（53.6 ±2.2 ）%，收率为（72.1 ±3.7 ）% 。结论 该方法优于目前常用 的黄酮富集方法，且工艺简单，具有良好的应用前景。 关键词：苦参；总黄酮；生物碱；富集方法；聚酰胺-大孔树脂联用 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2011)10 - 1968 - 05 Enrichment of total flavonoids from Sophora flavescens with polyamide- macroporous resin 1 1 2 2 1 ZHANG Lei , LIAO Qiong-feng , XIE Zhi-yong , FENG Zhi-qiang , ZENG Yuan-er 1. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China 2. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510080, China Abstract: Objective To explore the enriching method for the total flavonoids from Sophora flavescens with polyamide-macroporous resin. Methods Polyamide adsorbed with sample was washed with water first to eliminate the impurities and then was loaded on the top of macroporous resin. The total flavonoids were enriched by eluting with ethanol. Results AB-8 resin combined with polyamide could effectively enrich the total flavonoids and separate them thoroughly from the alkaloid compounds. The preferred condition for the procedure was as following: the weight of polyamide and dry AB-8 resin was one and three times of the extract weight (1:1 and 1:3), respectively. The volume of 80% ethanol for elution was 3 times of the column volume. The purity of total flavonoids reached to (53.6 ± 2.2)% and the recovery rate was (72.1 ± 3.7)%. Conclusion The method is not only superior