五种海洋生物葡萄糖磷酸变位酶和磷酸葡萄糖异构酶的同工 - 水产学报.pdf

30 2 V ol 30 , No 2 2006 4 Apr , 2006 JOURNAL OF FISHERIE S OF CHINA : 1000- 0615( 2006) 02- 0156- 05 1 1, 2 1 1 王伟继, 杨翠华 , 孔 杰, 王清印 ( 1 , 266071; 2 , 266003) : ( Strongylocentrotus intermedius) (Apostichopus j ap onicus Selenka) ( Cynoglossus semilaevis)(Meretrix meretrix) (Pecten yessoensis) ( PGM ) ( GPI) , PGM , GPI PGM GPI , PGM , GPI PGM , GPI PGM GPI , PGM GPI , 5 , , : ; ; ; ; ; ; ; : S917 : A Isozyme analysis of PGM and GPI in five marine organisms 1 1, 2 1 1 WANG We-i ji , YANG Cu-i hua , KONG Jie , WANG Qing- yin ( 1. Yellow Sea isheries ResearchInstitute, ChineseAcademy of ishery Sciences, Qingdao 266071, China; 2. Marine Lfi e College, Ocean University of China, Qingdao 266003, China) Abstract:Horizontal starch gel electrophoresis w as used to detect the genetic variation of PGM and GPI in f ive m arine organism s, sea urchin( Strongylocentrotus intermedius) , sea cucumber ( Apostichop us j aponicus Selenka ) , sole ( Cynoglossus semilaevis) , h ard clam ( Meretrix meretrix) and scallop ( Pecten yessoensis) For sea urchin , the PGM show ed high expression activity in muscle and m ature gonad and the loci number w as diff erent in the tw o tissues, w hile the activity of GPI w as rather low The isozym e demonstrated the sam e number of lo ci in the muscle and intestines of sea cucumber, but their activit