白果蛋白过敏动物模型的试验研究 - 中国农业科学.pdf

中国农业科学 2010,43(17):3616-3623 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.17.016 白果蛋白过敏动物模型的试验研究 杨剑婷，吴彩娥，李莹莹，贾韶千，范龚健，潘红梅 （南京林业大学森林资源与环境学院，南京 210037 ） 摘要：【目的】对白果蛋白致敏的小鼠模型进行研究，以明确白果蛋白是否具有致敏性。【方法】以Tris -HCl 缓冲液为阴性对照、卵白蛋白为阳性对照，设置白果蛋白低剂量组和高剂量组，每隔 7 d 经口灌胃致敏 1 次共 3 次，末次致敏7 d后腹腔注射激发，致使小鼠过敏。【结果】经白果蛋白及阳性对照激发后的小鼠血清产生高水平 IgE 及 IgG 抗体，体外激发小鼠致敏肥大细胞组胺释放率显著高于阴性对照，免疫期间血浆中组胺也显著升高， 小鼠肠、肺、肝均见炎症病灶，高剂量处理及阳性对照的肾脏也呈炎症细胞浸润。【结论】该模型可用于研究白果 蛋白致小鼠发生的IgE介导的Ⅰ型过敏反应。 关键词：白果；蛋白；过敏；小鼠；模型 Study on Animal Model of Allergy Provoked by Ginkgo Kernel Protein YANG Jian-ting, WU Cai-e, LI Ying-ying, JIA Shao-qian, FAN Gong-jian, PAN Hong-mei (Department of Forest Source and Environment, Nanjing Forest University, Nanjing 210037) Abstract: 【Objective 】 Mouse model of allergic reaction induced by ginkgo kernel protein was studied for the allergen assessment. 【Method 】 Taking Tris-HCl buffer solution as negative control and ovalbumin as positive control, different group of mice were sensitized by two doses of ginkgo kernel protein and controls orally on days 0, 7 and 14 d, and challenged intraperitioneally at 7 d after the last sensitization. 【Result 】 The result showed there were high level of IgE and IgG in ginkgo kernel protein group and positive control group, histamine release rate after challenged in vitro was higher significantly than the negative control. During the immune time, histamine in plasma was increased. There were inflammatory focuses in intestines, lungs and livers in mice treated with thinner dose of ginkgo kernel protein, and also in kidneys of mice treated with thicker dose of gingko kernel protein and the p