1 实验方法 - 中国比较医学杂志.doc

Rag2 KO小鼠脏器、血液生理生化指标及免疫的 王宇，吴旭颖杨志伟 （北京华阜康生物科技股份有限公司，北京102200） 【摘要】目的 Knockout（KO）小鼠的主要脏器重量，血生理生化指标和免疫细胞水平等生物学指标。方法 分别选5、10周龄的Rag2 KO 小鼠测定主要脏器重量，并测定主要血生理、生化指标选取6周龄Rag2 KO 小鼠，应用流式细胞仪检测细胞免疫（T细胞-CD3+、T细胞亚群-CD4+、CD8+)、体液免疫（B细胞-CD19+、B220+）、NK细胞（NK1.1+）和粒细胞（CD11b+）进行检测。结果同性别NOD/SCID小鼠10周小鼠脑、肺脏、脾脏、肝脏、心脏、双肾脏重TBIL、WBC高于5周龄。同周龄的雌雄相比，心脏、双肾脏、肝脏、脾脏重量AST、ALP、A/G、GLUPLT、PCT、WBC、LYM%高于雌性。Rag2 KO小鼠无T细胞（0.36±0.15）%、CD4+T细胞（0.21±0.06）%、CD8+T细胞（0.23±0.07）%、CD19+ B细胞（0.28±0.04）%和B220+ B细胞（2.03±0.42）%）。NK细胞比例为（24.13±3.62）%，粒细胞比例为（57.20±3.85）%。结论 Rag2 KO小鼠与C57BL/6J小鼠的主要生物学特性基本一致。Rag2 KO小鼠T、B细胞。【关键词】Rag2 K小鼠；脏器重量；血生理生化指标；免疫】 【文献标识码】】 【基金项目】 科技部“重大新药创制”科技重大专项——创新药物研究开发技术平台建设（） 【作者简介】，女，硕士，，第1作者 【通讯作者】@。The study on organ weights, blood physiological and biochemical parameters and immune cells in Rag2 KO mice Wang Yu, WU Xu-ying, Wei Zhao-sheng, WU Jin-feng, Liu Hui-zhen, Tian Yu-shu,ZHANG Ya-jing, ZHANG Wen-ming, YANG Zhi-wei (Beijing HFK Bioscience Co., LTD, Beijing 102200, China) 【Abstract】 Objective To bserved the organ weights, blood physiological and biochemical parameters, and immune function of Specific Pathogen Free (SPF) Rag2 knockout (KO) mice. Methods (1) Rag2 KO mice were selected at five and ten weeks ,and the organ weights, blood physiological and biochemical parameters were observed. The percentages of CD3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+ were checked by FCM in Rag2 KO mice at six week of age in terms of its T, B lymphocyte function and NK cell activity. Results Among the same sex group of NOD/SCID mice, the weights of brain, lung, spleen, liver, heart, kidney, and the levels of TBIL, WBC in 10 weeks of age are higher than 5 weeks. At the same age, the weights of heart, kidney, liver, spleen, and the levels of AST, ALP, A/G, GLU, PLT, PCT, WBC, and LYM% in male mice are higher than females. The Rag2 KO mice lacks T cells（0.36±0.15）%、CD4+ T cells（0.21±0.06）%、CD8+ T cells（0.23±0.07）%、CD19+ B cells（0.28±0.04）% 、B220+ B cells（2.03±0.42）%）. The percentage of