复方血栓通对1型糖尿病大鼠氧化应激的影响及 - 第三军医大学学报.doc

复方血栓通对1型糖尿病大鼠氧化应激的影响及机制探讨 邢玉微2，邹俊杰1，石勇铨1，刘志民1 (200003 上海市，解放军第二军医大学长征医院内分泌科1；050000 石家庄市，石家庄市第二医院内分泌科2) [提要] 目的 观察复方血栓通胶囊（XST）减轻糖尿病大鼠肾脏的损伤，探讨其保护机制。方法 通过腹腔注射链脲佐菌素streptozotocin STZ) 60mg/kg制作糖尿病大鼠模型, 成模大鼠随机分为糖尿病组(DM组,只)、糖尿病α-硫辛酸干预组(组,只)、糖尿病干预组(组,只)另设正常对照组(只)。组大鼠予以α-硫辛酸(0.1gkg)灌胃组大鼠予(1g/kg)灌胃DM组和组体积蒸馏水灌胃。测定肾皮质中超氧化物歧化酶(Superoxide dismutaseSOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase，GSH-Px)活性脂质过氧化产物丙二醛(malonic dialdehydeMDA)和诱导型一氧化氮合酶（iNOS）的mRNA含量。结果 和正常组相比，DM，XST，ALA组24h尿蛋白 (P0.05)，左肾重/体质量比（P＜0.01），尿白蛋白/肌酐比（P＜0.05），尿素氮（p＜0.01）升高，GSH-Px降低(P0.0)，MDA升高(P0.01,P0.05)LXS组和组肾皮质GSH-Px活性增加(P均0.01)MDA含量降低(P0.0,P0.05)。 [关键词] 糖尿病肾病；氧化应激；中药复方；硫辛酸，iNOS Effect of fufang xueshuantong on renal oxidative stress in rat model of type 1 diaetes mellitus and its possible mechanism Xing Yuwei2,Zou Junjie1,Shi Yongquan1,Liu Zhimin1 1、Department of Endocrinology, Changzheng Hospital, Sencond Military Medical University, Shanghai 200003,China 2、Department of Endocrinology，The second hospital of shijiaz，shijiaz，050000 [Abstract] Objective To observate effect of fufang xueshuantong on kidney in diabetic rat，and explore the protection mechanism。Methods After intraperitoneal injection streptozotocin (STZ)with 60mg/kg,diabetic rats were successfully induced.40 Rats were randomly divided into the following four groups equally: The normal control group(NG);fufang xueshuantong treatment group(XST);a-lipoic acid treatment group(ALA).After 12 weeks treatment, body weight,glucose, kidney weight/body weight(KW/BW),24h-urinary protein excretion, urea nitrogen,urinary albumin/creatinine ratio were compared。The level of super oxide dismutase SOD（Superoxide dismutase),MDA（malonic dialdehyde）,GSH-Px（Glutathione peroxidas） in renal cortical and the expression of iNOS of renal cortical by real time PCR were analysised。Results Compared with NG group,rats in DM group，XST group，ALA group had a higher 24 hour-urinary and KW/BW(P0.05)，urinary albumin/creatinine ratio(P＜0.05),activity of MDA(P0.01,P0.05)，expression of iNOS(