转bt-cry1ah基因玉米花粉对龟纹瓢虫解毒酶和 - 生物安全学报.doc

转ry1Ah基因玉米花粉对龟纹瓢虫解毒酶和中肠蛋白酶活性的影响王振营*，何康来，白树雄 （中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京 100193） 摘要：研究了取食转Bt-ry1Ah基因玉米花粉对龟纹瓢虫体内解毒酶和中蛋白酶活性的影响。结果发现，在解毒酶中取食Bt玉米花粉的龟纹瓢虫4龄和蛹期α-乙酸萘酯酶酶活性取食非Bt玉米花粉的龟纹瓢虫，取食Bt玉米花粉的龟纹瓢虫乙酰胆碱酯和谷胱甘肽-S-转移酶活性在各个发育时期与对照相比均没有显著差异。在中蛋白酶方面与对照组相比，取食Bt玉米花粉的龟纹瓢虫总蛋白酶和强碱性类胰蛋白酶的酶活性在各个发育时期均没有显著差异。但取食Bt花粉的龟纹瓢虫弱碱性类胰凝蛋白酶和类胰凝乳蛋白酶酶活性在蛹期时取食非Bt玉米花粉的龟纹瓢虫。 关键词：转Bt-ry1Ah基因玉米；花粉；龟纹瓢虫；解毒酶；中肠蛋白酶；酶活性Effects of transgenic cry1Ah corn pollen on the activity of some detoxification enzymes and midgut protease in Propylaea japonica (Thunberg) (Coleoptera: Coccinellidae) CUI Lei, WANG Zheng-Ying*, He Kang-Lai, Bai Shu-Xiong （State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193） Abstract: The experiments were conducted in laboratory to explore the effects of transgenic Bt- cry1Ah corn pollen on Propylaea japonica (Thunberg), mainly including the activtiy changes of detoxification enzymes and midgut proteinase in P. japonica reared on transgenic Bt-cry1Ah corn pollen. The results indicated that there were no differences between the activities of acetylcholinesterase, glutathione-S-transferase, total protease and active alkaline trysin-like proteinase in P. japonica at all instars fed on transgenic Bt-cryAh corn pollen and those on non-Bt pollen. The activity levels of α-naphthylacetate esterase in P. japonica at 4th instar and pupa, and the weak alkaline trysin-like proteinase and chymotrypsin-like proteinase in P. japonica at pupa fed on transgenic Bt-cry1Ah corn pollen was notably lower than that in the control. Key words: Transgenic Bt-cry1Ah corn; pollen; Propylaea japonica; detoxification enzyme; midgutprotease; enzyme activity 基金项目：转基因生物新品种培育科技重大专项（2008ZX08011-003） 作者简介：崔蕾（1983-），女，辽宁省沈阳市人，硕士，研究方向为转基因植物环境安全评价, E-mail: cuileigiggs@163.com *通讯作者， E-mail：zywangzy@。 随着转基因技术的飞速发展以及转基因玉米的大面积推广，在关注转基因玉米所带来的巨大的社会、经济和生态效益的同时，转基因玉米及其产品的安全性问题也引起了世界范围内的广泛的关注 et al.,1998; Crawley et al.,