低聚木糖生产废渣纤维素酶诱导和水解特性的研究 - 林产化学与工业.PDF

25 3 Vo.l 25No. 3 2005 9 Chem istry and Industry of Forest Products Sept. 2005 * 勇 强, 范一民, 徐 勇, 余世袁 (南京林业大学 化学工程学院, 江苏 南京 210037) : 研究了生产低聚木糖( XOS)所 的废渣对里氏木霉纤维素酶合成的诱导作用和纤 维素酶水解特性废渣对里氏木霉合成纤维素酶的诱导作用较差, 而纤维素酶水解性能优异 里氏木霉以含纤维素 15 g/L的废渣为碳源合成纤维素酶, 滤纸酶活为 0. 48 FPIU/mL, 酶产率 ONG Q 为 6. 67FPIU/ (L h), 酶 率为每克纤维素 32. 00 FPIU, 而在相同条件下以玉米芯为碳源时 滤纸酶活为 3. 20FPIU /mL酶产率 19. 00FPIU/ (L h)和酶 率每克纤维素 213. 33FPIU质量浓度为20 g/L 的废渣在酶用量为每克纤维素 10FPIU 条件下水解 24 h, 水解 率达 92. 8%; 底物废渣质量浓度为 100 g/L 时, 48 h纤维素酶水解 率达到 80. 6% : 低聚木糖; 纤维素酶; 酶水解;废渣处理 : TQ352. 78; Q556 : A : 0253- 2417( 2005) 03- 0001- 04 CHARACTERISTICS OF CELLULASE INDUCEMENT AND H DROL SIS B RESIDUE OF X LOOLIGOSACCHARIDE PRODUCTION ONG Qiang, FAN i-m in, XU ong, U Shi-yuan ( Co lleg e of Chem ical E ng ineering, N anj ing F orestry Un iv ersity, N anj ing 210037, Ch ina) A bstrac t: The characteristics of cellulase inducement and hydrolysis by residue of xylooligosaccharide ( XOS) productionwere investigated. The residue ofXOS production was sensitive to cellulase, but itwas a poor inducer for cellulase biosynthesis. The filter paper activity, volumetric productivity and yield by Tr ichod erm a r eese i, which employed residue of XOS production containing cellulose 15 g/L as carbon source, were 0. 48FPIU/mL, 6. 67 FPIU /( L h) and 32 FPIU/g cellulose, respectively. Compared to those of 3. 20 FPIU/mL, 19. 00 FPIU/ ( L h) and 213. 33 FPIU/g cellulose, which used untreated corncobs as carbon source. The