四种添加物对铁皮石斛原球茎生长及多糖含量的影响 - 热带亚热带植物 .pdf

热带亚热带植物学报　2014 ，22(3) ：257 ~ 262 Journal of Tropical and Subtropical Botany 四种添加物对铁皮石斛原球茎生长及 多糖含量的 影响 1,2 1* 2 包英华 , 潘超美 , 白音 (1. 广州中医药大学中药学院， 广州 510006； 2. 韶关学院英东生命科学学院， 广东 韶关 512005) 摘要：为探讨铁皮石斛(Dendrobium officinale)培养基 中添加物的作用 ，在 1/2MS 培养基 中加入椰 肉、甘蔗渣 、香蕉皮和麦麸等 4 种添加物 ，研究不 同浓度添加物和培养时间对原球茎生长和多糖含量 的影响。结果表明，4 种添加物对铁皮石斛原球茎 的增殖 、 分化和多糖含量均有一定影响 ，其 中添加 15.0 g L– 1 甘蔗渣 ，培养 60 d 能明显促进铁皮石斛原球茎 的增殖与分化(146.1%) ；而添 加 20.0 g L– 1 甘蔗渣 ，培养 40 d 能显著提高铁皮石斛原球茎多糖含量(50.4%) 。这说 明甘蔗渣是培养铁皮石斛原球茎 的适宜添 加物 ，既能促进铁皮石斛原球茎的生长发育 ，还能降低生产成本 。 关键词： 铁皮石斛 ；添加物 ；原球茎 ；多糖 doi: 10.3969/j .issn.1005–3395.2014.03.007 Effect of Four Additives on Growth and Polysaccharide Content of - Dendrobium officinale Protocorm like Bodies BAO Ying-hua1,2, PAN Chao-mei1*, BAI Yin2 (1. College of Chinese Material Medical, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; 2. Yingdong College of Life Sciences, Shaoguan University, Shaoguan 512005, China) Abstract: In order to understand the function of additive in medium of Dendrobium officinale, four additives, including coconut meat, sugarcane bagasse, banana peel and wheat bran, were supplied in 1/2MS medium, respectively. The effects of additives with different concentrates and culture time on growth and polysaccharide content of D. officinale PLBs were studied. The results showed that the PLBs proliferation, differentiation and the polysaccharide content were influenced by four additives. The proliferation rate of PBLs reached up to 146.1% supplemented with 15.0 g L–1 sugarcane bagasse and cultured for 60 d. The polysaccharide content of PLBs was increased for 50.4% supplemented with 20.0 g L–1 sugarcane bagasse and cultured for 40 days. So it was suggested that sugarcane bagasse was suitable additive for in vitro D. officinale PLBs owing to promote the growth of D. officinale PLBs and reduce cost of production. Key words: De