响应曲面法制备甘草次酸脂质体工艺优化 - 南京农业大学学报.pdf

摇 南京农业大学学报摇 2012,35(4):99-104 摇 Journal of NanjingAgricultural University http:/ / 試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試 赵晓娟,范云鹏,王梦琳,等.响应曲面法制备甘草次酸脂质体工艺优化[J].南京农业大学学报,2012,35(4):99-104 响应曲面法制备甘草次酸脂质体工艺优化 赵晓娟,范云鹏,王梦琳,赵翰飞,李彬,胡元亮,袁菊,王德云* (南京农业大学中兽医学研究室,江苏 南京210095) 摘要:为采用薄膜分散法制备甘草次酸脂质体,在单因素试验的基础上,采用响应曲面法研究了大豆磷脂与甘草次酸质量 比(脂药比)、大豆磷脂与胆固醇质量比(膜材比)和水浴温度3个因素对脂质体包封率的影响。 经过Design鄄Expert 7.0 软 件分析数据,得到脂质体的最佳制备条件:脂药比为9颐1,膜材比为2.5颐1,水浴温度为31 益。 在最佳制备条件下进行验证 试验,包封率实测值为(83.46依0.55)%,与包封率预测值相比,相对误差仅为0.47%。 结论:Box鄄Behnken Design结合响应 曲面分析法可以很好地对甘草次酸脂质体工艺进行优化。 关键词:甘草次酸脂质体;薄膜分散法;响应曲面法;制备工艺;包封率 中图分类号:S854.5摇 摇 摇 摇 文献标志码:A摇 摇 摇 摇 文章编号:1000-2030(2012)04-0099-06 Optimization on preparation condition of glycyrrhetinic acid liposome by response surface methodology ZHAO Xiao鄄juan,FAN Yun鄄peng,WANG Meng鄄lin,ZHAO Han鄄fei,LI Bin, HU Yuan鄄liang,YUANJu,WANG De鄄yun* (Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing210095,China) Abstract:Glycyrrhetinic acid(GA)liposomewaspreparedby thin鄄film dispersion method.Basedonthe single鄄factor experiment,the influence of ratio of soyben phosphatide to drug,ratio of soyben phosphatide to cholesteral and water bath temperature on the entrapment rate(ER)was studied by response surface methodology.The data was analyzed with Design鄄Expert 7.0 software,the optimum preparationconditionswereasfollows:ratioof soybenphosphatidetoGAwas9颐1,ratioof soybenphosphatidetocholesteral was 2.5颐1,and water temperature was 31 益.Verification tests were carried out under the optimal preparation condition and the average of ER was(83.46依0.55)%.Compared with the predicted value,the relative error was only 0.47%.The Bo