gpr30受体激动剂g-1对高糖诱导内皮细胞内质网应激的抑制作用.doc

G1对eahy926内皮细胞内质网应激的抑制作用 夏东晖1，吴士文2，袁明 3 （1.新乡医学院，新乡市 453003；2.中国人民武装警察部队总医院，北京市100039；3.中国航天员科研训练中心，北京市 100094） 【摘要】目的：观察GPR30受体激动剂G1对高糖诱导的eahy926内皮细胞内质网应激（endoplasmic reticulum stress，ERS）的影响。方法：选用eahy926内皮细胞为研究对象，分为3组：正常对照组（Con，17.51mmol/L）、高糖组（HG，33.3mmol/L）、高糖+G1组（HG+G1,HG+1umol/L G1），利用流式细胞术检测三组细胞凋亡率，Western blot法检测ERS相关分子BIP、IRE1、PERK及凋亡分子BAX、Bcl-2的表达变化，RT-PCR法检测BIP和CHOP的mRNA表达变化。结果：HG组与Con组比较，细胞凋亡率明显升高（P0.01），BIP、IRE1、PERK及凋亡分子BAX表达上调（P0.01或P0.001），Bcl-2的表达下调（P0.05），BIP、CHOP mRNA 表达上调（P0.01），HG+G1组与HG组比较，细胞凋亡率明显降低（P0.01），BIP、IRE1、PERK及凋亡分子BAX表达下调（P0.05或P0.01或P0.001），Bcl-2的表达上调（P0.05），BIP、CHOP mRNA 表达下调（P0.01）。结论：GPR30受体激动剂G1可抑制eahy926内皮细胞内质网应激。 【关键词】 G1；内质网应激；动脉粥样硬化；eahy926内皮细胞 G1 on eahy926 endothelial cell inhibition of endoplasmic reticulum stress XIA Dong-hui1，WU Shi-wen2，YUAN Ming3 （1.Xinxiang Medical University，Xinxiang， 453003，China； 2.Chinese Peoples Armed Police General Hospital，Beijing，100039，China；3.China Astronaut Research and Training Center，Beijing，100094，China；） 【Abstract】 Objective: To observe the effect of GPR30 agonist G-1 on high glucose-induced eahy926 endothelial cell endoplasmic reticulum stress (ERS). Methods: eahy926 endothelial cells for the study were divided into three groups: normal control group (Con, 17.51mmol / L), high glucose (HG, 33.3mmol / L), high glucose +G1 group (HG +G1, HG +1umol / L G1), cell apoptosis rate was measured by flow cytometry, Western blot assay ERS related molecules BIP, IRE1, PERK and apoptotic molecules BAX, Bcl-2 expression changes, RT-PCR assay the mRNA expression of BIP and CHOP. Result: Compared with Con group, the apoptosis in HG group was significantly increased (P 0.01), BIP, IRE1, PERK and apoptotic molecule BAX were upregulation (P 0.01 or P 0.001), Bcl-2 downregulation (P 0.05), BIP, CHOP mRNA expression increased (P 0.01), Compared with the HG group, apoptosis rate in HG +G1 group was significantly lower (P 0.01), BIP, IRE1, PERK and apoptotic