裸花紫珠提取物凝血有效部位的研究 - 环球中医药杂志.pdf

环球中医药2015年11月第8卷第11期摇 Global Traditional Chinese Medicine,November2015,Vol郾8,No郾11 摇1359 ·论著 · 裸花紫珠提取物凝血有效部位的研究 张利摇 黄胜摇 颜小捷摇 杨子明摇 谷陟欣摇 李典鹏 揖摘要铱摇 目的摇 研究裸花紫珠提取物凝血作用及其有效部位。 方法摇 以质控血浆为研究对 象,通过测定裸花紫珠粗提物及各分离部位和单体对凝血酶原时间(prothrombin time,PT)、凝血酶 时间(thrombintime,TT)、活化部分凝血活酶时间(activatedpartialthromboplastintime,APTT)、血浆纤 维蛋白原(fibrinogen,FIB)四项指标观察其对凝血系统的影响。 结果摇 与溶媒对照比较,裸花紫珠 粗提物能明显缩短PT、APTT、TT 的时间,能明显提高FIB含量;裸花紫珠40%甲醇洗脱部位能明显 缩短APTT、TT 的时间,能明显提高FIB含量;与粗提物比较,40%甲醇洗脱部位效果最接近于粗提 物。 结论摇 裸花紫珠有较好的凝血效果,其有效部位可能在40%甲醇洗脱部位。 揖关键词铱摇 裸花紫珠;摇 有效部位;摇 凝血;摇 凝血酶原时间 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2015郾11郾021 Effects ofactivefractionfromCallicarpanudiflora onbloodcoagulationsystem摇 ZHANGLi,HUANG Sheng, YAN Xiao鄄jie, et al. 摇 Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization,GuangxiInstitute of Botany,ChineseAcademy of Sciences,Guilin541006,China Corresponding author:LI Dian鄄peng,E鄄mail:ldp@ 揖Abstract铱摇 Objective摇 To study the effect of activefractionfrom Callicarpa nudiflora onblood co鄄 agulation system. Methods摇 The effect of active fraction from Callicarpa nudiflora on blood coagulation systemwereobservedwithprothrombintime(PT),activatedpartialthromboplastintime(APTT),thrombin time(TT) andfibrinogen(FIB)oftheconstituentsbloodsamplesweretested. Results摇 Comparedwiththe blank control,Callicarpa nudiflora extract could significantly lessen PT,TT,APTT,and could obviously increased the content of FIB;40% Methanol elution par could significantly lessen TT,APTT,and could obviously increased the content of FIB. Conclusion摇 The hemostatic effect of active fraction from Callicarpa nudiflora may be40% Methanol elution par of Callicarpa nudiflora. 揖Key words铱摇 Callicarpa nudiflora;摇 Hemostatic;摇 Mice;摇 Prothrombin time 摇 摇 裸花紫珠Callicarpa nudiflora Hook.et Arn.为马 散瘀消肿之功效,主治急性传染性肝炎、化脓性炎症、 鞭草科紫珠属植物,又名紫珠,主产于中国海南,是海