固相包被技术及应用.pdf

CNIC-01779 IAE-0211 固相包被技术及应用 王 衍 真 燕 强 奋 周 棱 郭 永 刚 贺 丰 (中国原子能科学研究院，北京，102413) 摘 要 对免疫分析中的生物分子包被固相载体技术做了系统的研究 ， 并探索其在免疫分析中的应用。采用物理吸附法和多聚赖氨酸活化 管化学连接方法对多克隆抗体和单克隆抗体在聚苯乙烯试管上的 固 定方法进行了研究，并用商品化的免疫分析试剂盒对研究的方法进 行了评估。采用物理吸附方法包被的试管稳定性较好，应用于TS H 和 CA125免疫放射分析，技术指标均达到国家暂行标准。采用活化 管技术包被的试管在应用某些具体的免疫分析试剂盒时，部分技术 指标高于核行业标准，但需进一步的实验 。 关键词：固相包被抗体活化管放射免疫分析免疫放射分 析 185 Technology of Antibody Immobilization on Solid Phase and its ，Application in Immunoassay (In Chinese) W A N G Yanzhen Y A N Qiangfe n Z H O U Len g GU O Yonggan g H E Youfen g (China Institute of Atomi c Energy , Beijing , 102413) ABSTRAC T Th e technology of biomolecul e immobilization o n soli d phase materials is studied and its? application in immunoassay is exploited . Th e polystyren e tube s were treated with poly-lysin e t o mak e th e surfac e functionalized . Th e poly - or mono-clonal antibodie s wer e immobilize d o n the surface—functionalized polysty - rene tube s via covalent attachment . Th e antibody coate d tube s wer e also pre - pared by passive adsorption . Th e antibody-attache d tube s prepare d with th e tw o kind of procedur e wer e evaluated with respect to their application in immu - noassay,. Th e passive adsorption procedur e fo r antibody coatin g o n polystyren e tube s wa s established.