小麦抗病品系5r625 抗叶锈病基因的分子鉴定 - 作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2015, 41(4): 651657 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2015.00651 小麦抗病品系5R625 抗叶锈病基因的分子鉴定 秦金燕 1 李在峰2 闫晓翠1 苏集华1 姚占军1,* 刘大群2,* 1 / , 071001; 2 / , 071001 : 5R625 , 36 15 5R625 , 5R625 15 Lr9 Lr19 Lr24 Lr28 Lr39 Lr47 Lr51 Lr53 5R625 5389 F 1F2 F2:3 5R625 , 5R625 1 F2:3 3DL 5R625 5 , STS 24-16 SCAR OP-J09 Lr24 , , 5R625 Lr24 : ; ; Lr24 ; SSR ; Molecular Identification of Leaf Rust Resistance Gene in Wheat Line 5R625 1 2 1 1 1,* 2,* QIN Jin-Yan , LI Zai-Feng , YAN Xiao-Cui , SU Ji-Hua , YAO Zhan-Jun , and LIU Da-Qun 1 College of Agronomy, Agricultural University of Hebei / North China Key Laboratory for Germplasm Resources of Education Ministry, Baoding 071001, China; 2 College of Plant Protection, Agricultural University of Hebei / Biological Control Center for Plant Disease Pests of Hebei Province, Baoding 071001, China Abstract: Wheat line 5R625 shows high resistance to all of Puccinia triticina pathotypes at seedling and adult plant stages, but its resistance mechanism is unclear. In this study, the resistance gene(s) were postulated by artificially inoculating 15 P. triticina pathotypes at the seedling stage and comparing infection types with those of 36 wheat lines with known Lr genes. The result showed that 5R625 might carry Lr9 , Lr19 , Lr24 , Lr28 , Lr39 , Lr47 , Lr51 , and Lr53. Further genetic analysis to confirm the resis- tance gene(