生防菌哈茨木霉fjat-9040固体发酵培养基的响应面优化 - yzxzcom.doc

以微生物发酵床养猪垫料为主要基质的哈茨木霉FJAT-9040固体发酵培养基优化 曾庆才1，2，肖荣凤2，刘 波2*，胡桂萍2 （1福州大学生物科学与工程学院，福建 福州 350108；2福建省农业科学院农业生物资源研究所，福建 福州 350003） 摘 要： 关键词：哈茨木霉；固体发酵；培养基优化 中图分类号： Optimization of solid-state fermentation culture medium consisted of the microbial fermentation bedding for the biocontrol strain of Trichoderma harzianum by response surface methodology Zeng Qing-cai1,2 Xiao Rong-feng2 Liu BO2* Hu Gui-ping2 College of Biological Science and Technology FuZhou University FuZhou FuJian 350108 China, 2.Agriculture Bioresource Research Institute, FuJian Academy of Agriculture Science, FuZhou,FuJian 350003 China) Abstract: Optimization of solid-state fermentation culture medium for a biocontrol strain Trichoderma harzianum FJAT-9040 using single factor analysis, Box-Behnken design.and response surface methodology (RSM). The medium mainly contained pig litter, which was produced from microbial fermentation bed for raising pigs, and the sporulation yield was used as the indicator in all tests.. The results of single factor tests showed that the ratio of pig litter and wheat bran, the content of wheat grain, carbon and nitrogen were 9:1, 40.00% of wheat grain, 3.00% of sucrose, and 2.00% of ammonium sulfate, respectively. The results showed that wheat grain, sucrose and ammonium sulfate were significant for the spore of Trichoderma harzianum based on the response surface methodology analysis(P0.05).The optimum medium constitutes were as followed: 9:1 of bedding of microbial pig raising and wheat bran,wheat grain 36.74%, sucrose 3.07%, ammonium sulfate 1.75%, and the average yield of spore reached to 2.98×109cfu·g-1 ,.which was closed to the actual fermentation. Key words: Trichoderma harzianum; Solid-state fermentation; Culture medium; Sporulation yield; Response surface methodology 木霉菌 Trichoderm广泛分布于土壤中，生命力强，适应性广，对许多植物病原真菌有拮抗作用。据不完全统计，对18个属29种植物病原真菌有拮抗作用。因此，木霉菌作为一类具有重要生防的生防而倍受关注。木霉，哈茨木霉T. harzianum)、绿色木霉T. viride）、钩状木霉T. hamatum）、康宁木霉T. koninngii）等。哈茨木霉Tharzianum的研究与应用，哈茨木霉T23、T158