大豆苷及大豆苷元溶解吸收特性差异比较及相互 - 国际中医中药杂志.pdf

国际中医中药杂志2015年5月第37卷第5期 Int J Trad Chin Med, May 2015, Vol. 37, No.5 ·439 · ·论著 · 大豆苷及大豆苷元溶解吸收特性 差异比较及相互作用研究 董红环 潘艳丽 刘洋 张雷 马晓云 罗志强 杨立媛 杨瑞瑞 【摘要】 目的 考察大豆苷和大豆苷元的溶解吸收特性及两者共存条件下在溶解和吸收方面的相 互影响。方法 采用溶解度实验技术测定大豆苷和大豆苷元的平衡溶解度；采用大鼠在体单向肠灌流方 法研究大豆苷元和大豆苷肠渗透性相互作用。结果 在 pH ＝7.4 的 K-R 试液中，大豆苷的溶解度约为大 豆苷元的 6 倍，两者共存时溶解度均有所增强。大豆苷元在大鼠肠道内的吸收系数约为大豆苷的 3 倍， 大豆苷对大豆苷元的吸收有显著性增强作用，但大豆苷元对大豆苷的吸收有抑制作用。结论 大豆苷元 的溶解度低于大豆苷，渗透性高于大豆苷。两者合用后大豆苷元的溶解度和渗透性均明显增强，大豆苷 的溶解度增强不显著，且渗透性显著降低。 【关键词】 大豆苷元；大豆苷；溶解度；渗透性；相互作用 The solubility, permeability and their interaction of daidzin and daidzein * Dong Honghuan , Pan Yanli, Liu Yang, Zhang Lei, Ma Xiaoyun, Luo Zhiqiang, Yang Liyuan, Yang Ruirui. Beijing University of Chinese Medicine, Beijing 100102, China Corresponding author: Liu Yang, Email: liuyang@bucm.edu.cn 【Abstract 】 Objective The aim of this study was to determine the solubility and permeability of daidzin and daidzein and the interaction of these two components. Methods With the method in Chinese Pharmacopoeia and in situ single-pass intestinal perfusion model we tested the solubility and permeability of daidzin, daidzein and their interaction. Results In pH 7.4 K-R buffer the solubility of daidzin was 6 times than daidzein and both the solubility of these two components were enhanced when they were determined together. In small intestine of rat, the permeability of daidzein was 3 times than daidzin. Daidzin could enhance the permeability of daidzein but the daidzein manifested an opposite trend. Conclusion When compared to daidzin, daidzein owned a lower solubility but a better permeabilit