property and application of bacy-based functional hydrogels 以二 .pdf

Property and Application of BACy-Based Functional Hydrogels 以二( 丙烯醯基)胱胺為基質之功能性水膠的性質與應用 1 2 1 *林宴夙 林照雄 李文福 1 大同大學化學工程系 2國立陽明大學生命科學系暨基因體科學研究所 Email: wflee@ttu.edu.tw Abstract Conventional polyacrylamide hydrogels Keywords prepared from radical polymerization between N, N-bisacrylylcystamine (BACy); Disulfide acrylamide and N,N-methylenebisacrylamide linkage; N, N-methylenebisacrylamide (NMBA) have been frequently used in the (NMBA); SDS biochemical technique like the sodium dodec- yl sulfate polyacrylamide gel electrophoresis 關鍵字 (SDS) to separate proteins. In this 二( 丙醯胺)胱胺; 雙硫鍵結 ; 二甲基雙丙醯 study, we have prepared an alternative 胺 ; 鈉十二烷基硫酸鹽聚丙烯醯胺凝膠電 polyacrylamide hydrogel from the cross- 泳 linking of acrylamide and N,N-bisacrylyl- cystamine (BACy). In addition, we have Introduction compared the BACy-based hydrogel with the SDShas been the frequently used NMBA-based polyacrylamide hydrogel for method in biochemical analysis and provides their physical properties such as swelling the capability to resolve complex protein ratio