萃取溶剂对当归根抗氧化性之影响 - 大仁科技大学.pdf

大仁學報第 47 期 萃取溶劑對當歸根抗氧化性之影響 15 Tajen Journal Vol. 47 萃取溶劑對當歸根抗氧化性之影響 吳天祚 * 蕭思玉**a 摘要 當歸 (根是國人常食用的藥草之一。本研究係探討不同的萃取溶劑對當歸) 根抗氧化性之影響。研究結果顯示萃取溶劑對當歸根抽出物之產率、總多酚含 量和抗氧化性有顯著地影響。以 乙醇和水溶劑萃取之抽出率和總多酚含量顯著 高於超臨界二氧化碳流體萃取者。在抗氧化性方面，所有抽出物之抗氧化活性 均呈現濃度效應 。乙醇和水抽出物之清除超氧陰離子自由基能力、還原力和螯 合亞鐵離子之能力皆顯著高於超臨界二氧化碳流體萃取抽出物 。但是在脂質過 氧化作用之抑制能力，超臨界二氧化碳流體萃取抽出物優於乙醇和水抽出物 。 整體而言，當歸根顯示出良好的抗氧化性，尤其在抑制脂質過氧化作用之能力 和清除超氧陰離子自由基之能力。 關鍵字 ：當歸，抗氧化性，多酚，溶劑 *大仁科技大學食品科技 系副教授 **大仁科技大學食品科技系教授 a 通訊作者 16 大仁學報 2015 年9月 15~28頁 第 47 期 Effect of extraction solvent on the antioxidant activity of dong-gui (the root of Angelica sinensis) Tien-Tso Wu* Sy-Yu Shiau **a Abstract Dong-gui is one of important traditional Chinese herbs. The objective of this study is to investigate that the effect of extraction solvent on the antioxidant activity of dong-gui (the root of Angelica sinensis). Results showed that extraction solvents, including 95% ethanol, water and supercritical CO2 fluid (SCF), significantly affected the extraction yield, total phenolic content and antioxidant activity of dong-gui. Both extraction yield and total phenolic content by ethanol and water were higher than those by SCF. All extracts exhibited the antioxidant activity in a concentration-dependent manner. Superoxide anion scavenging ability, reducing power and chelating-Fe2+ effect of both ethanol- and water- extracts were obviously higher than those of SCF extract. However, SCF extract had better inhibition of lipid peroxidation than ethanol- and water- extracts. Generally, dong-gui had good antioxidant activity, especially inhibition of lipid peroxidation and scavenging abil