3土壤线虫三种分离方法效率比较毛小芳.pdf

生态学杂志Chinese Journal of Ecology 　2004,23(3):149～ 151 　　　　　　　　　　　　　　　　　　　　　　　　　　 土壤线虫三种分离方法效率比较＊ ＊＊ 毛小芳　李辉信 　陈小云　胡　锋 (南京农业大学资 与环境科学学院农业资 与生态环境研究所, 南京 210095) 摘　要　比较了蔗糖离心浮选法、改进的贝尔曼漏斗法和浅盘法对土壤线虫的分离效率。蔗 糖离心浮选法分离得到的线虫总数分别为贝尔曼漏斗法的6.1 倍和浅盘法的11.4 倍。而且离 心浮选法分离得到的虫体大于53μm(300 目)和53～40μm(300 ～400 目)的线虫数显著高于 贝尔曼漏斗法和浅盘法。离心浮选法对所有功能类群线虫(包括食细菌、食真菌、植食和杂食 线虫)的分离效率也均显著高于其他2 种方法。贝尔曼漏斗法的分离效率高于浅盘法, 主要反 映在食细菌线虫和虫体在40～32μm(400 ～500)的线虫数。但在达到相同分离效率时, 浅盘法 比贝尔曼漏斗法需要的时间短。 关键词　土壤线虫, 分离效率, 蔗糖离心浮选法, 贝尔曼漏斗法, 浅盘法 中图分类号　S154　　　文献标识码　A 　　　文章编号　1000-4890(2004)03-0149-03 Extraction efficiency of soil nematodesby different methods.MAO Xiaofang, LI Huixin, CHEN Xi- aoyun, HU eng (Colleg e of R esour ces and Env ir onmental Sciences, Inst it ute of Ag r ic ult ur al Re - so ur ces and Eco-E nv ironment , N anj ing A gr icult ur al Univ ersity , Nanj ing 210095, Ch ina ).Ch inese Jour nal of Ecology , 2004,23(3):1～. Extraction efficiency of soil nematodesby density (sucrose)centrifugation, modified Baermann funnel and shallow dish methods w as compared.The total numbersof nematodes extracted by centrifugation method w ere 6.1 and 11.4 times higher than those by Baermann funnel and shallow dish methods, respectively.Nematodes of body size more than 53 μm(300 mesh)and between 53μm and 40 μm (300 ～400 mesh)extracted by centrifugation method were also significantly higher than those by Baermann funnel and shallow dish methods.In addition, centrifugation method w as more effective for