Dil散射标记神经元及神经胶质细胞技术介邓锦波.pdf

37 5 Vol137,No15 200610 ACTA ANATOMICA SINICA Oct. 2006 ## Dil 1, 2* 1 1 邓锦波 于东明 吴萍 ( 11 , 475001; 21Depart ent of Neuroscience, Brown University, USA) [] DiI (DiI diolistic assay) , , C57PB6J ,DiI , Dil ,, [] ; ; ;DiI ; C57PB6J [] Q189 [ ] A [ ] 0529-1356(2006)05-596 INTRODUCTION OF DiI DIOLISTIC ASSAY TO LABEL THE NERVOUS CELL AND GLIA 1, 2* 1 1 DENG Jin-bo , YU Dong- ing , WU Ping ( 11Institute of Neurobiology , Medical College of Hecnan University , K afi eng 475001, C ina; 21Department of Neuroscience, Brown University , USA) [Abstract] Objective The diolistic assay has been odified to ake it si pler and ore efficient in labeling neurons and glia. Meth ds CNS neurons andglial cellswerelabeledwith DiI diolistic assay in fixedtissue and livingbrain slices of C57PB6J ice. Results The ethod allowed thevisualization of thefine structure of neurons andglia including synaptic structures such as dendritic spines. C nclusi n Withthe ethod, the labeling efficacy of cell. s fine structure is i proved, aking it preferable for the analysis of dendritic spine. In addition, the ability to label the living neuron and gliawill extend its application vastly. [Key w rds] Neurons; Glia; Live-i aging; DiI diolistic labeling; C57PB6J ouse , ,Golgi , DiI , EGFP-DNA, DiI ( 1, 1c-