利用赤星病菌毒素快速高通量鉴定烟草抗性种质的方法 - 中国烟草科学.PDF

80 Chinese Tobacco Science 2014-0235 1 1,2 1* 1,2 1,2 3 1* 1. 266101 2. 100081 3. 266101 Nicotiana tobacum L. Beinhart1000-1NC89 G140 3 Beinhart1000-1NC89G140K326G28 100 r=-0.9588 S572.08 1007-5119201401-0080-05 DOI 10.13496/j.issn.1007-5119.2014.01.015 Investigation on the Method of Rapid Identifying Resistant Tobacco Germplasms to Alternaria Alternata by Using its Mycotoxins 1,2 1* 1,2 1,2 3 1* SUN Liping , SHI Jiao , MENG Kun , LUO Zhenning , ZHANG Junquan , WANG Fenglong (1. Tobacco Research Institute of CAAS, Qingdao 266101, China; 2. Graduate School of CAAS, Beijing 100081, China; 3. Qingdao Cigarette Factory of China Tobacco Shandong Industrial Co., Ltd, Qingdao 266101, China) Abstract: In order to find out an approach to rapidly identify resistant tobacco germplasm to Alternaria alternata , tobacco varieties Jingyehuang, Beinhart1000-1, NC89 and G140 were used as test materials. Using toxins produced by brown spot fungus (Alternaria alternata), we carried out a comparative test to choose a better screening method. Three kinds of screening methods were designed: toxins liquid soaking root of tobacco, toxins liquid soaking seeds of tobacco, and toxin-containing medium screening method. To verify the reliability of the toxin-containing medium screening method, Correlation between laboratory test results and field data on tobacco varieties Jingyehuang, Beinhart1000- 1, NC89, G140, K326, G28 and Zhongyan100 was analyzed, and correlation coefficient of -0.9588 was found, which suggests the effectiveness of laboratory method is close to those of field. Keywords: tobacco; brown spot disease; mycotoxin; identifying