钩藤碱通过抑制Smad 信号通路磷酸化减轻腹腔粘连发生的作用机制.PDF

药学学报 Acta Pharmaceutica Sinica 20 17, 52 (2): 229 235 ·229 · 藤碱通过抑制 Smad 信号通路磷酸化减轻腹腔粘连发生的作用机制 * 宇, 张 慧, 刘瑞丽, 海广范, 王 童, 岳佳新, 张炜丽, 任禹珂 (新乡医学院 学院, 河南 新乡 453000) 摘要: 腹腔粘连是临床上术后常见的并发症, 目前临床上还没有较好的针对性药物, 临床防治主要依靠物 理手段或激素。钩藤碱是传统中药钩藤的主要有效成分。本课题观察了钩藤碱对实验性腹腔粘连的防治效果, 实 验结果显示, 钩藤碱可剂量依赖地减轻腹腔粘连的程度, 显著降低大鼠血清白介素-6 (interleukin-6, IL-6) 、白介 素-1 (interleukin-1 , IL-1 ) 、肿瘤坏死因子-α (tumor necrosis factor-α, TNF-α) 腹腔液中转化生长因子- 1 (transforming growth factor- 1, TGF- 1) 和结缔组织生长因子 (connective tissue growth factor, CTGF) 的蛋白含 量。表明其防治腹腔粘连作用的机制可能与抑制炎症因子和TGF- 1/Smad 信号通路有关。 关键词: 钩藤碱; 腹腔粘连; 转化生长因子 1; 结缔组织生长因子 中图分类号: R963 文献标识码: A 文章编号: 0513-4870 (20 17) 02-0229-07 Prevention of abdominal adhesions in rats by rhynchophylline through inhibition of Smad singnaling pathway * SONG Yu , ZHANG Hui, LIU Rui-li, HAI Guang-fan, WANG Tong, YUE Jia-xin, ZHANG Wei-li, REN Yu-ke (College of Pharmacy, Xinxiang Medica l University, X inxiang 453000, China ) Abstract : Postoperative intra-abdominal adhesion is one of the most common complications in the postop- erative period. Current remedies are very ineffective to prevent the pathological outcomes except steroid hormones. Rhynchophylline is deemed as a pharmacologically active component from traditional Oriental medicine Uncaria rhynchop hylla (Miq.) Jacks. (Rubiaceae) . This study was designed to investigate the preven- tative effect of rhynchophylline on the abd