蜡蚧轮枝菌昆明菌株可湿性粉剂的发酵培养条件及其毒力测定张亚平.pdf

DOI :10.16409/ki.2095 -039x.2007.s1.005 23()28-31 　　　　　Chinese Journal of Biological Control　　　　　　　2007 9 蜡蚧轮枝菌昆明菌株可湿性粉剂 的发酵培养条件及其毒力测定 ＊ 张亚平, 徐启高, 杨美林 , 周天雄 (, 　650201) 摘要:采用价格低廉的农副产品结合液固两相培养的 法进行发酵培养, 干燥后的固体培养 基经粉碎后人工过300 目筛, 取菌粉(55%), 加过300 目筛的填料硅藻土(42%)、润湿剂十二 烷基苯磺酸钠(3%)及稳定剂混匀制得55%蜡蚧轮枝菌可湿性粉剂。该制剂有效孢子浓度 9 3.0×10 个/g;细度:97%以上过300 目筛;润湿时间:2′57″;悬浮率:75.6%;含水量 4 3.0%;pH 值6～7。该制剂对小菜蛾的LC 到8d 时为1.51×10 孢子/ml;在田间试验中,施 50 7 用3 次该可湿性粉剂2.1×10 孢子/ml 对萝卜蚜的15～25d 防治效果为64.9%～70.7%。 关　键　词:蜡蚧轮枝菌;可湿性粉剂;小菜蛾;毒力测定;萝卜蚜;田间药效试验 图分类号:S476.12　　文献标识码:A　　文章编号:1005-9261(2007)增刊-0028-04 Formulation Technology and Application of Verticillium lecanii Wettable Powder ZHAN Ya-ping, XU Qi-gao, YAN Mei-lin, ZHOU Tian-xiong (Institution of Plant Protection in Yunnan Agricultural University, Kunming 650201, China) Abstract:Using farm and sideline products as culture medium to cultivate Verticillium lecanii, then shatter it to obtain powder.55%powder, 42%diatomite, 3%SDBS and stabilizing agent constitute 9 55% V.lecanii wettablepowder.This formulations effective spores concentration is 3.0×10 spore/g; diameter of 97%of formulation is0.05 mm;wettabletime is 2min and 57 seconds;suspension percent- age is 75.6%;water capacity3.0%and its pH is 6-7.LC50 of thewettable powder to P lutella xy - 4 lostella can reach to 1.51×10 spore/mlin 8 d.Control efficiency of nimbin to L ip ap his erysimi is better than that of thewettable powder markedly. Key words:Verticillium lecanii ;wettable powder;Plutella xy lostella ;Lip ap his ery simi;bioassary [Verticillium lecanii