甜茶苷对变形链球菌致龋能力的影响.PDF

中草药 Chinese Traditional and Herbal Drugs 第45 卷 第12 期 2014 年6 月 ·1743 · 甜茶苷对变形链球菌致龋能力的影响 1 2* 胡 楠 ，熊兴耀 1. 湖南农业大学园艺园林学院，湖南 长沙 410128 2. 中国农业科学院蔬菜花卉所，北京 100081 摘 要：目的 研究甜茶苷对变形链球菌Streptococcus mutans 致龋能力的影响。方法 将变形链球菌菌液加入甜茶苷后于 恒温厌氧环境下培养，测试其pH 值变化、黏附率，运用碳氢化合物法测定其表面疏水率。采用Bradford 法以Neson-samogyi 分别测量总蛋白的量以及还原糖的量，计算葡萄糖基转移酶 （glucosyltransferase，GTF ）活力，采用Anihrone 法测量水不溶 性葡聚糖（water-insoluble glucans，WIG ）的量。结果 加入甜茶苷的菌液pH 变化值、黏附率、表面疏水率、GTF 活力及 WIG 的量与对照组之间均有显著性差异 （P ＜0.01 ）。结论 甜茶苷对变形链球菌产酸能力、黏附能力、GTF 活力及WIG 合 成具有显著抑制作用。 关键词：甜茶苷；变形链球菌；黏附；葡萄糖基转移酶；水不溶性胞外多糖；致龋能力 中图分类号：R285.5 文献标志码：A 文章编号：0253 - 2670(2014) 12 - 1743 - 04 DOI: 10.7501/j.issn.0253-2670.2014. 12.017 Effect of rubusoside on cariogenic potential of Streptococcus mutans 1 2 HU Nan , XIONG Xing-yao 1. College of Horticulture and Landscape, Hunan Agricultual University, Changsha 410128, China 2. Institute of Vegetables and Flowers, Chinese Academy of Agriculture, Beijing 100081, China Abstract: Objective To study the inhibitory effect of rubusoside on the growth of Streptococcus mutans. Methods S. mutans was added to each group, cultured in anaerobic incubator with constant temperature, and the pH value was measured and compared. Microbial adhesion to hydrocarbons method was used to test the adhesion and cell surface hydrophobicity. Centrifugation was followed, Bradford method and Neson-samogyi method were used to measure the total contents of protein and reducing sugar and to calculate the viability of glucosyltransferase (GTF). Anihrone method was used to measure the contents of water-insoluble glucans (WIG). Results There were highly significant differences among the sweet tea groups, the experiment groups, and control groups on the pH value, adhesion, cell surface hydrophobicity, GTF activity, and total content of WIG (P 0.01). Concl