毛竹PheDof4-1基因克隆及表达分析.PDF

安徽农业大学学报, 2017, 44(3): 398-403 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x010 网络出版时间：2017/5/24 11:18:53 [URL] /kcms/detail/34.1162.S1118.020.html 毛竹 PheDof4-1 基因克隆及表达分析 * 刘 俊，黄 容，程占超，牟少华，李雪平 ，高 健 ( 国际竹藤中心，国家林业局竹藤科学与技术重点实验室，北京 100102) 摘 要：Dof （DNA binding with one finger ）蛋白是植物特有的一类转录因子，由多基因共同编码，参与植物转 录调控、生长发育及胁迫响应等生物学过程。以毛竹为研究对象，克隆了1 个 Dof 基因，命名为 PheDof4-1 ，基因长 度为 1 473 bp ，推测其蛋白质产物的分子量是 53.2 kDa ，等电点为 8.05 。qRT –PCR 结果显示，PheDof4-1 在低温 和 250 mmol·L-1 NaCl 处理的幼茎中诱导表达，而在 20% PEG8000 处理的根中表达则受到强烈抑制，在叶片中不同 处理条件下呈现不同的表达趋势。研究结果为 PheDof4-1 在非生物胁迫中的作用提供理论依据，为竹子分子育种提 供参考。 关键词：毛竹；Dof 转录因子；非生物胁迫；表达模式 中图分类号：S795.7 文献标识码：A 文章编号：1672352X (2017)03039806 Molecular cloning and expression analysis of the PheDof4-1 gene in moso bamboo (Phyllostachys edulis) LIU Jun, HUANG Rong, CHENG Zhanchao, MU Shaohua, LI Xueping, GAO Jian (Key Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102) Abstract: Dof (DNA binding with one finger) proteins forming an important transcriptional factor family en- coding by multiple genes are involved in gene transcriptional regulation, development and stress-responses in plants. In this study, one of Dof genes, PheDof4-1 (Gene ID No. P1040) was isolated from moso bamboo and the sequence was 1 473 nt in length. The predicted molecular weight of PheDof4-1 protein was 53.2 kDa, and the pI value was 8.05. The qRT-PCR analysis displayed that the PheDof4-1 transcription level in young stem was in- duced following exposure to cold stress and salt treatment, however, t