党参皂甙对肾移植免疫排斥作用的实验研究 - 第三军医大学学报.docVIP

党参皂甙对肾移植免疫排斥反应作用的实验研究 张应添 何波 赵海 林涛 重庆医科大学附属儿童医院泌尿外科 重庆 400014 摘　要: 目的　研究党参皂甙对大鼠肾移植急性排斥反应的作用。方法　采用原位大鼠肾移植模型，共设3个实验组：假手术组（SH）、生理盐水对照组（NS）、党参皂甙组（SA）；根据观察时间点的不同又分别分3个亚组：3天组、5天组和8天组，于相应的时间点观察各组大鼠移植肾粘附分子ICAM-1的表达、受体脾脏IL-2的表达及移植肾排斥反应发生的程度。结果　党参皂甙应用于大鼠肾移植可使移植肾粘附分子ICAM-1表达增加，受体脾脏IL-2表达上升，可能引起移植肾排斥反应程度加重。结论　党参皂甙应用于肾移植可能加重移植肾的排斥反应，肾移植缺血－再灌注损伤期过后应慎用党参皂甙。 【关键词】：党参皂甙；肾移植；排斥反应 中图分类号儿童发育疾病研究省部共建教育部重点实验室lintao272@ tel:023作者介绍：张应添 硕士研究生 Email:asdf2681648@163.com Experiments of codonopsis saponin’s role in renal transplant immune rejection Ying-Tian Zhang, Bo He, Hai zhao, Tao Lin Department of Urology, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, 400014, China Address correspondence to Tao Lin, Department of Urology, ChongQing Chindren’s Hospital, ChongQing Medical University, No.136 Zhongshan 2nd Road, Yuzhong district, ChongQing 400014, China. E-mail: lintao272@ TEL: +86-023Fox: +86-023Abstract: Objective: To study the role in renal transplant immune rejection of codonopsis saponins. Methods: A model of renal transplantation in situ was used. All rats of experiment were divided into 3 groups: Sham operation group (SH),NS match group(NS), Codonopsis saponin intervention group (SA); According to the observation time points respectively devide into 3 different subgroups:3d group,5d group, 8d group; To the corresponding points in time observing the expression of each rat renal allograft adhesion molecules ICAM-1,the expressioon of receptor spleen IL - 2 and renal allograft rejection occurs degree of the renal allagraft. Result: codonopsis saponin could increase the expression of renal allograft adhesion molecules ICAM-1,and increase the expression of spleen IL-2,and may cause renal allograft rejection increasing. Concludsions: codonopsis saponin may increase the renal allograft rejection,so we had better stop to use codonopsis saponins after renal transplant ischemia-reperfusion injury period. Key words: