大鼠骨髓间充质干细胞通过膀胱壁注射改善大鼠逼 - 第三军医大学学报.doc

大鼠骨髓间充质干细胞通过膀胱壁注射改善大鼠逼尿肌无力 印苏培，郑 霁，董明明，周占松 （400037 重庆 第三军医大学西南医院全军泌尿外科研究所） [摘要] 目的 探讨大鼠骨髓间充质干细胞(rat bone-derived mesenchymal stem cells，rBMSCs)膀胱壁注射法改善大鼠逼尿肌无力(acontractile detrusor , ACD)的可能性。方法 40只SD雌性大鼠随机分为4组,每组10只：正常大鼠对照组、低温诱导ACD组、生理盐水治疗组、rBMSCs治疗组。常规分离培养rBMSCs至第三代。建立大鼠膀胱壁冻伤模型模拟逼尿肌无力的情况，将rBMSCs或生理盐水注入大鼠膀胱壁肌层，14 d后检测各组大鼠膀胱排尿功能，逼尿肌肌条收缩力，RT-PCR和Western blot检测平滑肌收缩相关蛋白（α-SMA、Calponin和SM-MHC）的表达情况，HE染色了解各组大鼠膀胱壁组织学改变情况。结果 rBMSCs治疗组膀胱内压、肌条收缩力显著高于低温诱导ACD组和生理盐水治疗组，恢复超过50%。治疗组α-SMA、Calponin及SM-MHC的表达明显高于低温诱导ACD组和生理盐水治疗组。结论 rBMSCs通过膀胱壁注射能有效改善大鼠逼尿肌无力。 [关键词] 骨髓间充质干细胞；平滑肌细胞；逼尿肌无力 [中图法分类号] [文献标志码] A Rat Bone Marrow-Derived Mesenchymal Stem Cells Improve Acontractile Detrusor in a Rat Model Yin supei,Zheng Ji,Dong mingming,Zhou zhansong (Urinary Surgey Research Insititute, Southwest Hospital, Third Military Medical University, Chongqing 400038, China) [Abstract] Objective To determine the possibility of improving acontractile detrusor (ACD) using cytotherapy with rat bone-derived mesenchymal stem cells (rBMSCs) injected into the wall of bladders. Methods Forty Sprague-Dawley female rats were divided into four groups (G). A control group of normal rats (G1, n = 10), and three other test groups of ACD rats: PBS as a negative control (G2, n = 10), Normal saline (NS) (G3, n = 10), rBMSCs (G4, n = 10). The rBMSCs were isolated and cultured as reported before. ACD was induced via cryoinjuried bladder. Isolated Cell injections in the G4 group occurred directly into the bladder wall. The implanted cells were tracked at 14 days post injection. Bladder micturition function, bladder detrusor strips tension, expression of smooth muscle contraction-related markers (α-SMA, Calponin and SM-MHC) and histological changes were assessed for each group. Result Compared with G2 and G3 group, the increased mRNA and protein expression levels of smooth muscle cell makers (α-SMA, Calponin and SM-MHC) in G4 group were detected using western blotting and reverse transcription-polymera