中药分析课程论文.doc

连翘药材中连翘苷的含量的测定及提纯 应用化学112班 王奔 摘要:建立连翘药材中连翘苷的含量测定方法，比较不同连翘药材中连翘苷的含量。方法:采用反相高效液相色谱法测定不同产地连翘药材中连翘苷的含量，流动相为乙睛一水(25 :75)，流速为1.OmL /min-1，检测波长为277nm，进样量为10 μL，柱温为30 0C。结果:在选定色谱条件下，连翘苷分离度良好，线性范围为0.4348一4. 348μg ， r = 0. 9999，平均回收率98.9%,RSD=0.68% (n=6);不同连翘药材中连翘苷含量差异较大，其中陕西洛南，湖北邵县以及四川成都的连翘药材中连翘苷含量相对高于其它产地。结论:该方法测定连翘中连翘苷的含量，简单、灵敏、准确、重现性好，连翘苷在各产地连翘间差异较大。 关键词:HPLC法;连翘;连翘苷 Abstract : Objective:To develop a method for determining the content of phillyrin in Fruc;tus Forsythiae and to c;om-pare the content in Fruc;tus Forsythiae from different habitats. Methods:HPLC was adopted to determine the content of phillyrin} the mobile phase was ac;etonitrile一water (25:75)，the velocity was 1.0mL·min-1，the UV detection wavelength was 277nm} the sampling volume was l0mL} the temperature of column was 300C.Results:Under the selected Chromatography condition the phillyrin was observed in a good separation degree ， a good linear with range of 0. 4348-4.348μg， r=0.9999，the average recovery was 98.9% and RSD was 0. 68%(n=6) . The content of phillyrin in Fructus Forsythiae from different habitats is different of which the content of phillyrin in Fruc;tus Forsythiae from Luonan( Shaanxi )，Shaoxian ( Hubei) and Chengdu ( Sichuan) are higher than that of others. Conclusion : The method is simple sensitive } ac;c;urate and with good reproducibility. It c;an he used to determine the content of phillyrin in Fruc;tus Forsythiae. The content is different with the habitat of Fruc;tus Forsythiae. Key words:HPLC;Habitat;tus Forsythiae;Phillyrin 引言：连翘为木犀科植物,为常用中药材,具有清热解毒、消肿散结的功效具有较强的抑菌和抗病毒，抗疲劳，减肥等作用。近年来从该植物果实中分离得到了连翘苷、连翘酯苷等有效成分，而对于连翘叶化学成分研究的较少。运用高效液相色谱法测定连翘叶不同提取物中连翘苷的含量，充分利用连翘叶资源，为天然抗氧化剂连翘苷资源的开发利用研究提供理论依据。 1 含量的测定 1.1、仪器 Hitachil二一7100高效液相色仪，Hitachil二一7420紫外一可见检测器，Hitachi D- 7000色谱工作站。连翘苷:购自成都思科华生物制品公司，纯度)9800(含量测定用)。 1.2方法与结果 1.2. 1色谱条件色谱柱:Phenomenex Ci、柱(250mm x4.6mm}5N,m) ;流动相:乙睛一水(25 : 75 ) ;检测波长:277nm;柱温:300C;流速:1.0mL·min-1;进样量:10μL;理论塔板数按连翘苷峰计算应不低于3000。该色谱条件下，连翘样品在连翘苷样品处无干扰， 1.2. 2对照品溶液的制备